J Vet Sci.  2021 Jan;22(1):e8. 10.4142/jvs.2021.22.e8.

Expression and evaluation of porcine circovirus type 2 capsid protein mediated by recombinant adenoassociated virus 8

• Li S
• Wang B
• Jiang S
• Lan X
• Qiao Y
• Nie J
• Yin Y
• Shi Y
• Kong W
• Shan Y

• KMID: 2512439
• DOI: http://doi.org/10.4142/jvs.2021.22.e8

Abstract

Background
Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide.
Objectives
A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy.
Methods
In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vitro after gene transfer.
Results
The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2.
Conclusions
The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.

Keyword

Porcine circovirus type 2; recombinant adeno-associated virus 8; capsid protein