Korean J Vet Res.  2011 Mar;51(1):7-14.

Quantification and genotyping of PCV2 DNA in the tissues of PCV2-infected conventional pigs with different clinical signs

• Kim HK
• Luo Y
• Moon HJ
• Park SJ
• Rho SM
• Han JY
• Nguyen VG
• Park BK

Abstract

This study was focused on the genotyping and quantification of Porcine circovirus type 2 (PCV2) in thirty PCV2-positive pigs with different clinical symptoms (PCV2-infected without wasting, PCV2-infected with wasting, PCV2-infected with wasting and lymphoid depletion). The quantity of PCV2 DNA in diverse tissues was significantly differed among these groups. (One-way ANOVA test, p < 0.001) Interestingly, PCV2-DNA load in tissues of PCV2-infected pigs without wasting and PCV2-infected pigs with wasting and lymphoid depletion were not significantly differed (p = 0.38), while they were all significantly higher when compared with PCV2-infected pigs with wasting-only. PCV2 DNA quantity in tissues was significantly higher in PCV2a and 2b co-infected pigs compared to the PCV2b only-infected pigs (Wilcoxon test, p = 0.039). The PCV2a and 2b co-infected pigs had increased wasting and lymphoid depletion rate but it was not statistically significant. Therefore, this cross-sectional study suggested that PCV2 DNA load in tissues was diverse by clinical and histological findings. Furthermore, co-infection of PCV2a and 2b affected to the PCV2 DNA load in tissues with increased rate of wasting and lymphoid depletion.

Keyword

clinical sign; genotype; PCV2a; PCV2b; Quantification

MeSH Terms

Circovirus
Coinfection
Cross-Sectional Studies
DNA
Genotype
Swine
DNA