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Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs

van Wyngaardt W, Mashau C, Wright I, Fehrsen J

There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody...
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Assessing PCV2 antibodies in field pigs vaccinated with different porcine circovirus 2 vaccines using two commercial ELISA systems

Shin MK, Yoon SH, Kim MH, Lyoo YS, Suh SW, Yoo HS

Porcine circovirus type 2 (PCV2) is the primary causative agent for post-weaning, multisystemic, wasting syndrome. Consequently, serologic detection of and vaccination against PCV2 are important for the swine industry. Among...
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Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine

Yang DK, Kim BH, Lim SI, Kwon JH, Lee KW, Choi CU, Kweon CH

The Japanese encephalitis virus (JEV) is one of causative agents of reproductive failure in pregnant sows. An indirect enzyme-linked immunosorbent assay (I-ELISA) was examined for its potential use in the...
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Development of immunoassays for the detection of kanamycin in veterinary fields

Jin Y, Jang JW, Han CH, Lee MH

Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be...
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ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera

Ahn HJ, Kim S, Kim DY, Nam HW

An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of...
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Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application

Lee DY, Cho YW, Kang SG, Shin SJ, Yoo HS

  • KMID: 1093629
  • J Vet Sci.
  • 2004 Dec;5(4):337-343.
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and...
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Evaluation of a competitive ELISA for antibody detection against avian influenza virus

Song DS, Lee YJ, Jeong OM, Kim YJ, Park CH, Yoo JE, Jeon WJ, Kwon JH, Ha GW, Kang BK, Lee CS, Kim HK, Jung BY, Kim JH, Oh JS

Active serologic surveillance is necessary to control the spread of the avian influenza virus (AIV). In this study, we evaluated a commercially-available cELISA in terms of its ability to detect...
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