Korean J Parasitol.  2003 Sep;41(3):175-177. 10.3347/kjp.2003.41.3.175.

ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera

Affiliations
  • 1Department of Parasitology and Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea.
  • 2Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.

Abstract

An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the Cterminal of the molecule was expressed as a 6 x His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals.

Keyword

Neospora caninum; cattle; IgG-ELISA; Nc-p43; recombinant antigen; Toxoplasma gondii

MeSH Terms

Animals
Antibodies, Protozoan
Cattle
Cattle Diseases/diagnosis/epidemiology
Coccidiosis/diagnosis/epidemiology/veterinary
Enzyme-Linked Immunosorbent Assay/methods/*veterinary
Immunoglobulin G/*analysis
Neospora/*immunology
Protozoan Proteins/*immunology
Seroepidemiologic Studies
Full Text Links
  • KJP
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr