Korean J Parasitol.  2000 Dec;38(4):245-249. 10.3347/kjp.2000.38.4.245.

Detection of IgG antibody against Neospora caninum in cattle in Korea

• Bae JS
• Kim DY
• Hwang WS
• Kim JH
• Lee NS
• Nam HW

• KMID: 1057181
• DOI: http://doi.org/10.3347/kjp.2000.38.4.245

Abstract

A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%) reacted positively to either Nc-1 or KBA-2 strains of N. caninum. Among the 113 positive sera, 92 sera (81.4%) reacted with antigens of both strains, but 6 sera (5.3%) with Nc-1 and 15 sera (13.3%) with KBA-2 strain only. And with T. gondii antigen, 6 sera (1.2%) were positive but all reacted with N. caninum antigen also. Western blot revealed typical binding pattern according to ELISA values, such that high OD group reacted specifically to the major surface proteins including 43 kDa protein. Seroprevalence of 23.0% indicates that neosporosis seemed to be one of major causes of abortion in cattle. It is suggested here to establish more epidemiological researches nationwide systematically.

MeSH Terms

Animal
Antibodies, Protozoan/blood*
Cattle
Cattle Diseases/epidemiology*
Coccidiosis/veterinary*
Coccidiosis/epidemiology
Enzyme-Linked Immunosorbent Assay
Human
IgG/blood*
Korea/epidemiology
Neospora/immunology*
Seroepidemiologic Studies
Toxoplasma/immunology
Toxoplasmosis, Animal/epidemiology