Korean J Parasitol.
2013 Feb;51(1):129-132. 10.3347/kjp.2013.51.1.129.
Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle
- Affiliations
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- 1Islamic Azad University of Jahrom, Jahrom, Iran.
- 2Razi Vaccine and Serum Research Institute, Shiraz, Iran. namavari@yahoo.com
- KMID: 1501821
- DOI: http://doi.org/10.3347/kjp.2013.51.1.129
Abstract
- In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.
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