Korean J Parasitol.  2013 Apr;51(2):247-253. 10.3347/kjp.2013.51.2.247.

Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum

• Jia LJ
• Zhang SF
• Qian NC
• Xuan XN
• Yu LZ
• Zhang XM
• Liu MM

• KMID: 1499707
• DOI: http://doi.org/10.3347/kjp.2013.51.2.247

Abstract

Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7, expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was 10(9)TCID50/ml. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 1:4,096. IFN-gamma and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.

Keyword

Neospora caninum; bovine, NcSRS2-NcGRA7; recombinant adenovirus, immunity

MeSH Terms

Adenoviridae/*genetics
Animals
Antibodies, Fungal/blood
Antigens, Fungal/genetics/*immunology
*Drug Carriers
Fungal Proteins/genetics/*immunology
Fungal Vaccines/administration & dosage/genetics/*immunology
Immunoglobulin G/blood
Interferon-gamma/blood
Interleukin-4/blood
Mice
Mice, Inbred BALB C
Neospora/genetics/*immunology
Recombinant Fusion Proteins/genetics/immunology
Vaccines, Synthetic/administration & dosage/genetics/immunology
Antibodies, Fungal
Antigens, Fungal
Drug Carriers
Fungal Proteins
Fungal Vaccines
Immunoglobulin G
Recombinant Fusion Proteins
Vaccines, Synthetic
Interleukin-4
Interferon-gamma