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Immunogenicity of a Canine Parvovirus 2 Capsid Antigen (VP2-S1) surface-expressed on Lactobacillus casei

Moon HJ, Chowdhury , Kim CJ, Shin KS

  • KMID: 1498927
  • J Biomed Res.
  • 2013 Jun;14(2):91-98.
Canine parvovirus (CPV2) is one of the most virulent virus causing acute hemorrhagic enteritis and myocarditis in dogs. Infection mainly caused by the ingestion of virus through the mucosal route....
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Properties of GST-CALM expressed in E. coli

Kim JA, Kim SR, Jung YK, Woo SY, Seoh JY, Hong YS, Kim HL

  • KMID: 1109309
  • Exp Mol Med.
  • 2000 Jun;32(2):93-99.
Clathrin-coated vesicles (CCVs) are involved in protein and lipid trafficking between intracellular compartments in eukaryotic cells. CCVs are composed of clathrin and assembly proteins. The clathrin assembly protein lymphoid...
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Detection of antibodies against glucose 6-phosphate isomerase in synovial fluid of rheumatoid arthritis using surface plasmon resonance (BIAcore)

Kim JY, Lee MH, Jung KI, Na HY, Cha HS, Ko EM, Kim TJ

  • KMID: 1109321
  • Exp Mol Med.
  • 2003 Aug;35(4):310-316.
We have used a surface plasmon resonance biosensor (SPR, BIACORE 2000) to detect antibodies against glucose 6-phosphate isomerase (GPI) in synovial fluids of rheumatoid arthritis (RA) and osteoarthritis (OA). Recombinant...
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Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum

Jia LJ, Zhang SF, Qian NC, Xuan XN, Yu LZ, Zhang XM, Liu MM

Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens...
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Distribution of Adenoviral Vector in Brain after Intravenous Administration

Jin JY, Moon CI, Moon CI, Kang WS, Jeong DC

The delivery of transgenes to the central nervous system (CNS) can be a valuable tool to treat CNS diseases. Various systems for the delivery to the CNS have been developed;...
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An antibody reactive to the Gly63-Lys68 epitope of NT-proBNP exhibits O-glycosylation-independent binding

Lee Y, Kim H, Chung J

The N-terminal fragment of prohormone brain natriuretic peptide (NT-proBNP) is a commonly used biomarker for the diagnosis of congestive heart failure, although its biological function is not well known. NT-proBNP...
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A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein

Song KJ, Yang Z, Chong CK, Kim JS, Lee KC, Kim TS, Nam HW

  • KMID: 1792140
  • Korean J Parasitol.
  • 2013 Oct;51(5):503-510.
Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can...
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Monitoring antibody titers to recombinant Core-NS3 fusion polypeptide is useful for evaluating hepatitis C virus infection and responses to interferon-alpha therapy

Park YM, Byun BH, Choi JY, Bae SH, Kim BS, So HS, Ryu WS

To evaluate the clinical feasibility of the antibody titer against a chimeric polypeptide (named Core 518), in which a domain of Core and NS3 of hepatitis C virus (HCV) was...
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Prokaryotic expression and characterization of human AP DNA endonuclease

Oh SH, Song DW, Lee MY

The expression of major human apurinic/apyrimidinic DNA endonuclease (APEX) from its cDNA in E. coli (DH5 alpha) was attempted in order to obtain a biologically active recombinant APEX....
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Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus

Lv L, Li X, Liu G, Li R, Liu Q, Shen H, Wang W, Xue C, Cao Y

Infectious bronchitis virus (IBV) poses a severe threat to the poultry industry and causes heavy economic losses worldwide. Vaccination is the most effective method of preventing infection and controlling the...
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Identification of Atg8 Isoform in Encysting Acanthamoeba

Moon EK, Hong Y, Chung DI, Kong HH

  • KMID: 1792139
  • Korean J Parasitol.
  • 2013 Oct;51(5):497-502.
Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cyst-forming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess...
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Characterization and epitope mapping of two monoclonal antibodies against human CD99

Gil MC, Lee MH, Seo JI, Choi YL, Kim MK, Jung KC, Park SH, Kim TJ

  • KMID: 1121094
  • Exp Mol Med.
  • 2002 Dec;34(6):411-418.
CD99 plays an critical role in the diapedesis of monocytes, T cell differentiation, and the transport of MHC molecules. Engagement of CD99 by agonistic monoclonal antibodies has been reported...
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Sterile Inflammation after Intravitreal Injection of Aflibercept in a Korean Population

Kim JY, You YS, Kwon OW, Kim SH

PURPOSE: To report the frequency and clinical features of sterile inflammation after intravitreal aflibercept injection in a Korean population. METHODS: A single-center, retrospective study was performed in patients who received intravitreal...
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Gene cloning of the human cytomegalovirus (HCMV) antigen reactive with the serum from a HCMV-infected patient

Kim MN, Park CG, Hwang ES, Lim DG, Park JW, Seoh JY, Kook YH, Lee HJ, Choi SB, Cha CY

The human cytomegalovirus(HCMV) gene encoding the protein reactive with the sera of HCMV-infected patient was cloned and characterized. A reactive phage clone was screened from a lambda gt11 expression library...
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Human SNF2L Gene Is Regulated Constitutively and Inducibly in Neural Cells via a cAMP-Response Element

Xia Y, Wang L, Ma C, Gong Y, Zhao Y

PURPOSE: SNF2L belongs to Imitation Switch family and plays an essential role in neural tissues and gonads. In our previous studies, we have demonstrated that the basal transcription of human...
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A Case of Successful Treatment of Cutaneous Aspergillosis with Voriconazole at the Low Cyclosporine Trough Level in a Renal Transplant

Park SJ

Aspergillosis is a serious infectious complication with high mortality in transplant recipients. Voriconazole is a broad spectrum triazole antifungal agent, but it has a drug-drug interaction with immunosuppressants. Herein we...
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Expression of Th1 and Th2 type cytokines responding to HBsAg and HBxAg in chronic hepatitis B patients

Lee M, Lee MA, Lee SK, Son M, Cho SW, Park S, Kim HI

The cytokine pattern on viral antigen recognition is believed to exert a profound influence on the resolution of viral infections and viral clearance. This study was initiated to investigate whether...
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T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity via alphavbeta3 and alpha5beta1 integrins

Nam JO, Jung MY, Thapa N, Lee BH, Park RW, Kim IS

We made fusion protein of fastatin and FIII 9-10, termed tetra-cell adhesion molecule (T-CAM) that can interact simultaneously with alphavbeta3 and alpha5beta1 integrins, both playing important roles in tumor angiogenesis....
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In vivo determination of the gap2 gene promoter activity in Giardia lamblia

Yang HW, Kim J, Yong TS, Park SJ

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase...
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Conjugation and deconjugation of ubiquitin regulating the destiny of proteins

Baek KH

  • KMID: 1088651
  • Exp Mol Med.
  • 2003 Feb;35(1):1-7.
The homeostasis for a number of cellular proteins is regulated by not only phosphorylation and dephosphorylation, but also ubiquitination and deubiquitination. A number of proteins involved in the degradation of...
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