Korean J Parasitol.  2006 Mar;44(1):21-26. 10.3347/kjp.2006.44.1.21.

In vivo determination of the gap2 gene promoter activity in Giardia lamblia

• Yang HW
• Kim J
• Yong TS
• Park SJ

• KMID: 1091832
• DOI: http://doi.org/10.3347/kjp.2006.44.1.21

Abstract

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.

Keyword

Giardia lamblia; gap2 gene expression; encystation; luciferase; transfection

MeSH Terms

Transfection/methods
Time Factors
Recombinant Fusion Proteins/analysis/biosynthesis
Promoter Regions (Genetics)/*physiology
Plasmids
Luciferases/genetics/metabolism
Life Cycle Stages/physiology
Giardia lamblia/*genetics
Genetic Engineering/methods
Genes, Reporter/genetics
Genes, Protozoan/genetics/*physiology
Gene Order
Gene Expression/genetics/*physiology
GTPase-Activating Proteins/*genetics
Blotting, Southern/methods
Animals