Korean J Parasitol.  2020 Dec;58(6):675-679. 10.3347/kjp.2020.58.6.675.

Functional Identification of a Nuclear Localization Signal of MYB2 Protein in Giardia lamblia

• Kim J
• Shin MY
• Park SJ

• KMID: 2510691
• DOI: http://doi.org/10.3347/kjp.2020.58.6.675

Abstract

MYB2 protein was identified as a transcription factor that showed encystation-induced expression in Giardia lamblia. Although nuclear import is essential for the functioning of a transcription factor, an evident nuclear localization signal (NLS) of G. lamblia MYB2 (GlMYB2) has not been defined. Based on putative GlMYB2 NLSs predicted by 2 programs, a series of plasmids expressing hemagglutinin (HA)-tagged GlMYB2 from the promoter of G. lamblia glutamate dehydrogenase were constructed and transfected into Giardia trophozoites. Immunofluorescence assays using anti-HA antibodies indicated that GlMYB2 amino acid sequence #507–#530 was required for the nuclear localization of GlMYB2, and this sequence was named as NLS_GlMYB2. We further verified this finding by demonstrating the nuclear location of a protein obtained by the fusion of NLS_GlMYB2 and G. lamblia glyceraldehyde 3-phosphate dehydrogenase, a non-nuclear protein. Our data on GlMYB2 will expand our understanding on NLSs functioning in G. lamblia.

Keyword

nuclear localization signal; GlMYB2