Korean J Parasitol.  1999 Jun;37(2):121-125. 10.3347/kjp.1999.37.2.121.

Axenic cultivation and characterization of Giardia lamblia isolated from humans in Korea

• Park SJ
• Yong TS
• Yang HW
• Lee DH
• Lee K

• KMID: 1715275
• DOI: http://doi.org/10.3347/kjp.1999.37.2.121

Abstract

Inoculating of human fecal cysts to suckling Mongolian gerbils, two Giardia lamblia isolates, K1 and K2, were established as axenic cultures. Using this in vitro culture, both isolates were characterized as a "medium-rate grower" upon its growth pattern. These two Giardia isolates were grouped by using two genetic analysis. With genetic analysis of SSU-rDNA sequences, both K1 and K2 were found as members of Hopkins' group 1, despite some nucleotide differences noticed in K2 (5 differences/292 bases). The other genetic study used PCR-RFLP of the tim (triose phosphate isomerase) gene. Both of K1 and K2 were found to belong to Nash's group 2. Our results suggest that Nash's group 2 can not be a separate group, but a part of Hopkins' group 1.

MeSH Terms

Animal
Base Sequence
Gerbillinae
Giardia lamblia/growth & development
Giardia lamblia/genetics*
Giardia lamblia/classification
Human
Korea
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length