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Analysis of the Vaginal Microbiome by Next-Generation Sequencing and Evaluation of its Performance as a Clinical Diagnostic Tool in Vaginitis

Hong KH, Hong SK, Cho SI, Ra E, Han KH, Kang SB, Kim EC, Park SS, Seong MW

BACKGROUND: Next-generation sequencing (NGS) can detect many more microorganisms of a microbiome than traditional methods. This study aimed to analyze the vaginal microbiomes of Korean women by using NGS that...
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Optimization of Protein Extraction for Lichen Thalli

Kondratiuk AS, Savchuk OM, Hur JS

Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the...
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Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea

Kim K, Yoon S, Cheun HI, Kim JH, Sim S, Yu JR

Microsporidia are eukaryotic organisms that cause zoonosis and are major opportunistic pathogens in HIV-positive patients. However, there is increasing evidence that these organisms can also cause gastrointestinal and ocular infections...
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Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum

Jia LJ, Zhang SF, Qian NC, Xuan XN, Yu LZ, Zhang XM, Liu MM

Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens...
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Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes

Kim JK, Park YJ, Kong WS, Kang HW

  • KMID: 2312600
  • Mycobiology.
  • 2010 Dec;38(4):331-335.
In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using...
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Improved Algorithms for the Identification of Yeast Proteins and Significant Transcription Factor and Motif Analysis

Lee SW, Hong SE, Lee KY, Choi DI, Chung HY, Hur CG

  • KMID: 2166220
  • Genomics Inform.
  • 2006 Jun;4(2):87-93.
With the rapid development of MS technologiesy, the demands for a more sophisticated MS interpretation algorithm haves grown as well. We have developed a new protein fingerprinting method using a...
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Localization of cytoskeletal proteins in Pneumocystis carinii by immuno-electron microscopy

Yu JR, Pyon JK, Seo M, Jung BS, Cho SR, Lee SH, Hong ST

Pneumocystis carinii causes serious pulmonary infection in immunosuppressed patients. This study was undertaken to observe the cytoskeletal proteins of P. carinii by immuno-electron microscopy. P. carinii infection was experimentally...
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Characterization of yeast deoxyhypusine synthase: PKC-dependent phosphorylation in vitro and functional domain identification

Kang KR, Chung SI

  • KMID: 1029929
  • Exp Mol Med.
  • 1999 Dec;31(4):210-216.
The biosynthesis of hypusine [Nepsilon-(4-amino-2-hydroxybutyl)-lysine] occurs in the eIF-5A precursor protein through two step posttranslational modification involving deoxyhypusine synthase which catalyzes transfer of the butylamine moiety of...
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