J Korean Med Sci.  2015 Mar;30(3):227-232. 10.3346/jkms.2015.30.3.227.

Detection of Encephalitozoon spp. from Human Diarrheal Stool and Farm Soil Samples in Korea

Affiliations
  • 1Department of Environmental and Tropical Medicine & Research Institute of Medical Sciences, Konkuk University, School of Medicine, Seoul, Korea. maria205@kku.ac.kr
  • 2Division of Epidemic Inteligence Service, Korea Centers for Disease Control & Prevention, Cheongju, Korea.

Abstract

Microsporidia are eukaryotic organisms that cause zoonosis and are major opportunistic pathogens in HIV-positive patients. However, there is increasing evidence that these organisms can also cause gastrointestinal and ocular infections in immunocompetent individuals. In Korea, there have been no reports on human infections with microsporidia to date. In the present study, we used real-time PCR and nucleotide sequencing to detect Encephalitozoon intestinalis infection in seven of 139 human diarrheal stool specimens (5%) and Encephalitozoon hellem in three of 34 farm soil samples (8.8%). Genotype analysis of the E. hellem isolates based on the internal transcribed spacer 1 and polar tube protein genes showed that all isolates were genotype 1B. To our knowledge, this is the first report on human E. intestinalis infection in Korea and the first report revealing farm soil samples as a source of E. hellem infection. Because microsporidia are an important public health issue, further large-scale epidemiological studies are warranted.

Keyword

Microsporidia; Encephalitozoon intestinalis; Encephalitozoon hellem; Real-time PCR; Diarrhea; Soil

MeSH Terms

AIDS-Related Opportunistic Infections/parasitology
Adolescent
Adult
Aged
Agriculture
Base Sequence
Child
Child, Preschool
DNA, Intergenic/genetics
DNA, Protozoan/genetics
Encephalitozoon/*genetics/*isolation & purification
Encephalitozoonosis/*epidemiology
Feces/*parasitology
Female
Fungal Proteins/genetics
Humans
Infant
Infant, Newborn
Male
Middle Aged
Molecular Typing
Real-Time Polymerase Chain Reaction
Republic of Korea/epidemiology
Sequence Alignment
Sequence Analysis, DNA
Soil/*parasitology
Young Adult
DNA, Intergenic
DNA, Protozoan
Fungal Proteins
Soil
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