J Bacteriol Virol.  2020 Mar;50(1):9-16. 10.4167/jbv.2020.50.1.009.

Age-specific Prevalence of Porcine Reproductive and Respiratory Syndrome Virus, Porcine Circovirus Type 2, and Mycoplasma hyopneumoniae in Korea Pig Farms

• Kang I
• Ham H

• KMID: 2471833
• DOI: http://doi.org/10.4167/jbv.2020.50.1.009

Abstract

Porcine respiratory disease complex (PRDC) continues to be a significant economic problem to the swine industry. Porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and Mycoplasma hyopneumoniae (MH) are considered to be the most important pathogens that cause PRDC. In this study, we investigated the prevalence of antibodies against PRRSV and MH in the serum of sows and piglets from 89 domestic commercial pig farms by ELISA, and the presence of viral nucleic acids of PRRSV, including North American and European PRRS, and PCV2 was also investigated in the serum of sows and piglets from 89 domestic commercial pig farms by real-time PCR. In case of PRRSV, 78.7% (70/89) of sows were positive for PRRSV antibody, and 96.6% (86/89) of piglets were positive for PRRSV antibody. For MH, 76.4% (68/89) of sows showed positive for MH antibody. In the PRRSV viral nucleic acid detection experiment, 36.0% (32/89) of sows were positive for PRRSV nucleic acids, and virus nucleic acid was detected in 83.1% (74/89) of piglets. In case of virus type, both North American and European types were detected. In case of PCV2, 15.7% (14/89) of sows were positive for PCV2 nucleic acids. Conclusively, PCV2, PRRSV, and MH were widely distributed in pig farms in Korea. These prevalence data related with PRDC provides clinical information for vaccination strategy and development for the control of PRDC.

Keyword

ELISA; real-time PCR; Mycoplasma hyopneumoniae; porcine circovirus type 2; porcine reproductive and respiratory syndrome virus; porcine respiratory disease complex

MeSH Terms

Agriculture*
Antibodies
Circovirus
Enzyme-Linked Immunosorbent Assay
Korea*
Mycoplasma hyopneumoniae*
Mycoplasma*
Nucleic Acids
Porcine Reproductive and Respiratory Syndrome*
Porcine respiratory and reproductive syndrome virus*
Prevalence*
Real-Time Polymerase Chain Reaction
Swine
Vaccination
Antibodies
Nucleic Acids

Reference

1). C Kang MS, Kang MW, Jung SH, Lee HS. Study on porcine respiratory disease complex from slaughtered pigs in Namwon, Korea. Korean J Vet Serv. 2013; 36:139–45.

2). Chae C. Porcine respiratory disease complex: Interaction of vaccination and porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, and Mycoplasma hyopneumoniae. Vet J. 2016; 212:1–6.

3). Lee CH, Hwang WM, Lee JG, Lee SM, Kim SJ, Kim NH, et al. Study on gross finding of lung lesions and causative pathogens of porcine respiratory disease complex from slaughtered pigs in Incheon. Korean J Vet Serv. 2011; 34:313–20.

4). Chu KS, Kang MS, Jo YS, Lee JW. Detection of porcine circovirus 2, porcine reproductive and respiratory syndrome virus and mycoplasma hyopneumoniae from swine lungs with lesions by PCR. Korean J Vet Serv. 2008; 31:71–7.

5). Jeong CG, Khatun A, Nazki S, Lee SI, Kim TH, Kim KS, et al. Production and evaluation of PRRS resistant pigs. Korean J Vet Serv. 2019; 42:1–7.

6). Gagnon CA, Del Castillo JR, Music N, Fontaine G, Harel J, Tremblay D. Development and use of a multiplex real-time quantitative polymerase chain reaction assay for detection and differentiation of Porcine circovirus-2 genotypes 2a and 2b in an epidemiological survey. J Vet Diagn Invest. 2008; 20:545–58.

7). Park KH, Oh T, Yang S, Cho H, Kang I, Chae C. Evaluation of a porcine circovirus type 2a (PCV2a) vaccine efficacy against experimental PCV2a, PCV2b, and PCV2d challenge. Vet Microbiol. 2019; 231:87–92.

8). Wasilk A, Callahan JD, Christopher HJ, Gay TA, Fang Y, Dammen M, et al. Detection of US, Lelystad, and European-like porcine reproductive and respiratory syndrome viruses and relative quantitation in boar semen and serum samples by real-time PCR. J Clin Microbiol. 2004; 42:4453–61.

9). Kang IJ, Kang HS, Jeong JW, Park CH, Kim SU, Choi KH, et al. Improved growth performance by type 2 porcine reproductive and respiratory syndrome virus (PRRSV)-based modified live vaccine in a herd with concurrent circulation of type 1 and type 2 PRRSV. Thai J Vet Med. 2017; 47:109–15.

10). Cheong Y, Oh C, Lee K, Cho KH. Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method. J Vet Sci. 2017; 18:283–9.

11). Oh T, Kim H, Park KH, Yang S, Jeong J, Kim S, et al. Comparison of 4 commercial modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines against heterologous Korean PRRSV-1 and PRRSV-2 challenge. Can J Vet Res. 2019; 83:57–67.

12). Park C, Jeong J, Choi K, Chae C. Efficacy of a new bivalent vaccine of porcine circovirus type 2 and Mycoplasma hyopneumoniae (Fostera ^TM PCV MH) under experimental conditions. Vaccine. 2016; 34:270–5.

13). Lee HH, Rha J, Han JH. Evaluation of vaccination against Mycoplasma hyopneumoniae in pigs at different vaccination time-points. Korean J Vet Serv. 2008; 31:291–303.

14). Hong SH, Lee HA, Kim DW, Kim TW, Kim OJ. Simultaneous diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections by multiplex PCR. Korean J Vet Serv. 2014; 37:247–52.

15). Choi WZ, Hong GS, Jeong WH, Kim NS, Kim NS, Kim KT, et al. Prevalence of porcine circovirus type 2 from slaughtered pigs in eastern area of Gangwon province. Korean J Vet Serv. 2006; 29:249–56.

16). Oh Y, Seo HW, Park C, Chae C. Comparison of sow and/or piglet vaccination of 3 commercial porcine circovirus type 2 (PCV2) single-dose vaccines on pigs under experimental PCV2 challenge. Vet Microbiol. 2014; 172:171–80.