J Vet Sci.  2013 Jun;14(2):115-124. 10.4142/jvs.2013.14.2.115.

Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea

• Choi EJ
• Lee CH
• Song JY
• Song HJ
• Park CK
• Kim B
• Shin YK

• KMID: 1705512
• DOI: http://doi.org/10.4142/jvs.2013.14.2.115

Abstract

The high genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) has been an obstacle to developing an effective vaccine for porcine reproductive and respiratory syndrome (PRRS). This study was performed to assess the degree of genetic diversity among PRRSVs from Korean pig farms where wasting and respiratory syndrome was observed from 2005 to 2009. Samples from 786 farms were tested for the presence of PRRSV using reverse transcription PCR protocol. A total of 117 farms were positive for type 1 PRRSV while 198 farms were positive for type 2. Nucleotide sequences encoding the open reading frame (ORF) 5 were analyzed and compared to those of various published PRRSV isolates obtained worldwide. Sequence identity of the ORF 5 in the isolates was 81.6~100% for type 1 viruses and 81.4~100% for type 2 viruses. Phylogenetic analysis of the ORF 5 sequences showed that types 1 and 2 PRRSVs from Korea were mainly classified into three and four clusters, respectively. The analyzed isolates were distributed throughout the clusters independent of the isolation year or geographical origin. In conclusion, our results indicated that the genetic diversity of PRRSVs from Korean pig farms is high and has been increasing over time.

Keyword

Korea; open reading frame 5; phylogenetic analysis; porcine reproductive and respiratory syndrome virus

MeSH Terms

Animal Husbandry
Animals
*Genes, Viral
*Genetic Variation
Lung/virology
Lymph Nodes/virology
*Open Reading Frames
Phylogeny
Porcine Reproductive and Respiratory Syndrome/virology
Porcine respiratory and reproductive syndrome virus/chemistry/classification/*genetics/isolation & purification
Republic of Korea
Reverse Transcriptase Polymerase Chain Reaction/veterinary
Sequence Analysis, DNA/veterinary
Sequence Analysis, Protein/veterinary
Swine

Figure

• 

  Fig. 1 (A) Phylogenetic analysis of the open reading frame (ORF) 5 in Korean type 1 porcine reproductive and respiratory syndrome viruses (PRRSVs). The phylogenetic tree was constructed with 117 PRRSV isolates from Korea and 46 PRRSV strains isolated from around the world. The VR-2332 strain was used as the outgroup. Gray boxes and bundle lines indicate genetic clusters (I, II, and III) of Korean isolates. Bootstrap values greater than 500 of 1,000 replicates are indicated. PRRSV strains are denoted as follows: name of the PRRSV strain/GenBank accession no./country name/collection time, year published, or vaccine. Isolates are denoted by serial numbers (Table 6). (B) Korean type 1 PRRSV isolates collected from 2007 to 2009 belonging to cluster I.

• 

  Fig. 2 (A) Phylogenetic analysis of the ORF 5 in Korean type 2 PRRSVs. The phylogenetic tree was constructed with 198 Korean PRRSV isolates and 57 PRRSV strains isolated from around the world. The LV was used as the outgroup. Gray boxes and bundle lines indicate genetic clusters (I, II, III, and IV) of Korean isolates. Bootstrap values greater than 500 of 1,000 replicates are indicated PRRSV strains are denoted as follows: name of PRRSV strain/GenBank accession no./country name/collection time, year published, or vaccine. Korean isolates are denoted by serial numbers (Table 7). (B) Korean type 2 PRRSV isolates between 2005 and 2009 belonging to cluster I. (C) Korean type 2 PRRSV isolates collected from 2005 to 2009 belonging to cluster II. (D) Korean type 2 PRRSV isolates obtained from 2005 to 2009 belonging to cluster III. (E) Korean type 2 PRRSV isolates acquired from 2005 to 2009 belonging to cluster IV.

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