J Vet Sci.  2009 Dec;10(4):309-315. 10.4142/jvs.2009.10.4.309.

Phylogenetic characterization of genes encoding for glycoprotein 5 and membrane protein of PRRSV isolate HH08

• Wang M
• Li G
• Yin J
• Ren X

• KMID: 1726903
• DOI: http://doi.org/10.4142/jvs.2009.10.4.309

Abstract

A porcine reproductive and respiratory syndrome virus (PRRSV) was obtained from clinic samples. Genes 5 and 6 encoding for the viral glycoprotein 5 and a membrane protein of the PRRSV designated as HH08 were amplified by reverse transcription-PCR. These sequences were compared with reference sequences derived from different geographical locations. The results indicated that the virus belongs to the North American type rather than European. Comparative analyses of the genetic diversity between the PRRSV isolate HH08 and other Chinese as well as foreign reference strains of PRRSV were discussed based on the sequence comparison and the topology of phylogenetic trees constructed in this study.

Keyword

GP5; M protein; phylogenetic analysis; PRRSV

MeSH Terms

Animals
Base Sequence
China/epidemiology
Gene Expression Regulation, Viral/physiology
Genetic Variation
Molecular Sequence Data
*Phylogeny
Porcine Reproductive and Respiratory Syndrome/epidemiology/virology
Porcine respiratory and reproductive syndrome virus/*genetics/*metabolism
Sequence Alignment
Swine
Viral Envelope Proteins/genetics/*metabolism
Viral Matrix Proteins/genetics/*metabolism