J Biomed Res.  2013 Jun;14(2):55-59.

T helper 1-type immunogenicity of Mycoplasma hyopneumoniae antigen on mouse spleen cells

• Yim SH
• Joo HG

Abstract

Mycoplasma hyopneumoniae (M. hyopneumoniae) is one of the causative bacteria that can induce chronic enzootic pneumonia, resulting in low production in the swine industry. Potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia by M. hyopneumoniae has also been recognized. Although some available vaccines have been developed for prevention of M. hyopneumoniae infection, protective immunity is still poor. In this study, in order to provide valuable information on vaccine antigen, we investigated the immunogenicity of M. hyopneumoniae on mouse spleen cells. Concanavalin A (ConA) and lipopolysaccharide (LPS) were used for generation of activated T and B lymphocytes. M. hyopneumoniae made clusters of spleen cells and also affected the cellular activity and viability of spleen cells by alone or with mitogens. Of particular interest, it induced a significant increase in production of TNF-alpha in ConA-treated spleen cells, meaning T helper 1 response. In addition, cell size and mitochondrial membrane potential of M. hyopneumoniae-treated spleen cells were measured by flow cytometric analysis. M. hyopneumoniae did not affect the cell size by alone, whereas ConA or LPS profoundly increased the cell size. Taken together, M. hyopneumoniae significantly affect the cellular activity and cytokine production of spleen cells by alone or in a combination of ConA. This study provides valuable information for production of the vaccine against M. hyopneumoniae.

Keyword

Mycoplasma hyopneumoniae; spleen cells; immunogenicity; T helper 1 response; vaccine

MeSH Terms

Animals
B-Lymphocytes
Bacteria
Cell Size
Concanavalin A
Membrane Potential, Mitochondrial
Mice*
Mitogens
Mycoplasma hyopneumoniae*
Mycoplasma*
Pneumonia
Porcine Reproductive and Respiratory Syndrome
Spleen*
Swine
Tumor Necrosis Factor-alpha
Vaccines
Concanavalin A
Mitogens
Tumor Necrosis Factor-alpha
Vaccines