Ann Dermatol.  2012 Feb;24(1):26-31. 10.5021/ad.2012.24.1.26.

Effect of IGF-I on Hair Growth Is Related to the Anti-Apoptotic Effect of IGF-I and Up-Regulation of PDGF-A and PDGF-B

• Ahn SY
• Pi LQ
• Hwang ST
• Lee WS

• KMID: 2156852
• DOI: http://doi.org/10.5021/ad.2012.24.1.26

Abstract

BACKGROUND
Insulin-like growth factor-I (IGF-I) shares a high degree of structural and functional homology with insulin and is a potent mitogen supporting cell growth and survival in many kinds of the tissues and cells. It also plays a role in some differentiation and anti-apoptotic functions. In previous reports, it has been shown that IGF-I stimulates hair follicle (HF) growth, maintains the anagen stage, and postpones the catagen stage.
OBJECTIVE
The exact mechanism of the effect of IGF-I on HF growth is not yet established. Therefore, we investigated the relationships between IGF-I and various other factors (i.e. apoptosis related molecules, pro-inflammatory cytokines, other growth factors, etc.) in the control of HF growth.
METHODS
The effect of IGF-I on human hair growth was measured using an organ culture model of human HFs and compared with a control group that did not receive IGF-I. We also measured mRNA expression of factors related to hair growth and apoptosis (which was determined by reverse transcription polymerase chain reaction (RT-PCR). RT-PCR was done on days 2, 4, 6, and 8 of organ culture.
RESULTS
In organ cultured human hair follicles, IGF-I had a positive effect on the rate of linear hair growth. IGF-I maintained the anagen phase. IGF-I increased the expression of platelet-derived growth factor (PDGF)-A, PDGF-B and the expression ratio of Bcl-2/Bax.
CONCLUSION
The effect of IGF-I on hair growth appears to be related to the upregulation of PDGF-A and PDGF-B and to the anti-apoptotic effect of IGF-I.

Keyword

Hair growth; IGF-I; PDGF-A; PDGF-B

MeSH Terms

Apoptosis
Cytokines
Hair
Hair Follicle
Humans
Insulin
Insulin-Like Growth Factor I
Intercellular Signaling Peptides and Proteins
Organ Culture Techniques
Platelet-Derived Growth Factor
Polymerase Chain Reaction
Reverse Transcription
RNA, Messenger
Up-Regulation
Cytokines
Insulin
Insulin-Like Growth Factor I
Intercellular Signaling Peptides and Proteins
Platelet-Derived Growth Factor
RNA, Messenger

Figure

• 

  Fig. 1 Quantitative analysis of hair shaft average elongation. Hair follicles were incubated in the presence or absence of insulin-like growth factor-I (IGF-I) for 12 days. The length of the hair follicles was measured in 2 day interval. The hair shaft average elongation of IGF-I group showed more greater compared to control group. This tendency was most prominent at 2nd days. Data are based on 24 hair follicles per group, and presented as the mean±SEM. *p<0.05.

• 

  Fig. 2 Effect of insulin-like growth factor-I (IGF-l) on cumulative hair follicle elongation for 12 days: The difference between two group was firstly identified at 2nd day of experiment. All day of experiment showed a statistical significance. *p<0.05.

• 

  Fig. 3 (A) In both group, until 4th day, all hair follicle remain anagen stage. on the 8th day, control group hair follicles showed more dermal papillae shrinkage than control group. (B) Insulin-like growth factor-I (IGF-I) treated group showed more anagen proportion from 6th day to 12th day of experiment.

• 

  Fig. 4 Results of reverse transcription polymerase chain reaction: IL-1α, IL-1β, INF-γ, TNF-α and TGF-β, did not show significant difference of their expression between two groups. But at 2 day of experiment, insulin-like growth factor-I (IGF-I) treated group show more prominent expression of PDGF-A & PDGF-B. Among the apoptosis related molecules, in 8th day, Bax was more weakly expressed, in IGF-I treated group and Bcl-2 showed more strongly expressed in second day of experiment. GAPDH: glyceraldehyde-3-phosphate dehydrogenase, IL: interleukin, TNF: tumor necrosis factor, INF: interferon, TGF: transforming growth factor, PDGF: platelet-derived growth factor.

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