J Korean Orthop Assoc.  2002 Apr;37(2):288-294.

Effect of Growth Factors on Type I Collagen Synthesis in Cultured Rabbit's Deep Flexor Tendon Cell

• Shin DE
• Kang HJ
• Kim HW
• Kang ES
• Hahn SB
• Kim SJ
• Han CD
• Lee WS

Abstract

PURPOSE: This study focuses on the change of the expression of type-I collagen mRNA and extent of collagen synthesis caused by four kinds of growth factos (TGF-b, IGF-I, EGF, PDGF) and their combinations to elucidate the effects of growth factors on rabbit's flexor tendon.
MATERIALS AND METHODS
Cells were isolated from rabbit's flexor tendons and cultured with DMEM containing 0.5% fetal bovine serum. Growth factors were then given either independently or in combination. On the 5th, 10th, and 15th day, the cells were collected and the cell proliferation rate was analyzed. The expression of type-I collagen mRNA was analyzed by Northern blot.
RESULTS
The expression of type-I collagen mRNA increased significantly in the IGF, PDGF, PDGF/EGF, and PDGF/IGF groups (p<0.05). Western blot analysis showed that the extent of type-I collagen synthesis in culture media was similar to the type-I collagen mRNA expression.
CONCLUSION
Based on this study, we conclude that PDGF and IGF individually, and the combinations PDGF/IGF and PDGF/EGF were most effective at aiding the healing process in rabbit's flexor tendon.

Keyword

Tendon cell culture; Growth factor; Type-I collagen; Rabbit flexor tendon

MeSH Terms

Blotting, Northern
Blotting, Western
Cell Proliferation
Collagen
Collagen Type I*
Culture Media
Epidermal Growth Factor
Insulin-Like Growth Factor I
Intercellular Signaling Peptides and Proteins*
RNA, Messenger
Tendons*
Collagen
Collagen Type I
Culture Media
Epidermal Growth Factor
Insulin-Like Growth Factor I
Intercellular Signaling Peptides and Proteins
RNA, Messenger