Ann Dermatol.  2011 May;23(2):150-155. 10.5021/ad.2011.23.2.150.

Effects of Human Adipose-derived Stem Cells on Cutaneous Wound Healing in Nude Mice

• Lee SH
• Lee JH
• Cho KH

• KMID: 2156655
• DOI: http://doi.org/10.5021/ad.2011.23.2.150

Abstract

BACKGROUND
Despite numerous treatments available for deteriorated cutaneous wound healing such as a diabetic foot, there is still the need for more effective therapy. Adipose-derived stem cells (ASCs) are mesenchymal stem cells, which are self-renewing and multipotent. Mesenchymal stem cells have the potential for tissue repair and regeneration.
OBJECTIVE
To investigate the effects of human ASCs on the healing of cutaneous wounds in nude mice.
METHODS
15-mm round full-thickness skin defects were generated on the back of BALB/c nude mice. The mice were divided into three groups for wound coverage: (i) human ASCs-populated collagen gel, (ii) human dermal fibroblasts-populated collagen gel, and (iii) collagen gel alone. Wound contraction was prevented with a splint method. Wound size was measured 10 days after injury. At 28 days histological analysis was performed.
RESULTS
Both ASCs and dermal fibroblasts accelerated wound closure, but dermal fibroblasts were more effective than ASCs. At 28 days, the dermal portion of ASCs or dermal fibroblasts wound scars were thicker than collagen gel wound scars.
CONCLUSION
ASCs and dermal fibroblasts stimulate cutaneous wound healing and improve scar thickness.

Keyword

Adipose tissue; Fibroblast; Mesenchymal stem cell; Mouse; Wound healing

MeSH Terms

Adipose Tissue
Animals
Cicatrix
Collagen
Contracts
Diabetic Foot
Fibroblasts
Humans
Mesenchymal Stromal Cells
Mice
Mice, Nude
Skin
Splints
Stem Cells
Wound Healing
Collagen

Figure

• 

  Fig. 1 Schematic representation of wound model. (A) Galiano's model. 6 mm round wounds were created on the mouse dorsum. The donut-shaped silicone sheet splint was centered on the wound, fixed with an immediate-bonding adhesive and interrupted nylon sutures. (B) Modified model used in our experiment. 15 mm wounds were made on the dorsal skin of mouse. A flexible plastic splint was fixed to the skin around the wound with an adhesive film and interrupted 6-0 nylon sutures.

• 

  Fig. 2 Both adipose-derived stem cells (ASCs) and dermal fibroblasts (DFs) accelerated wound closure, but DFs were more effective than ASCs. (A) BALB/c nude mice were wounded and treated with collagen gel alone (control) or ASCs or DFs in collagen gel. After 10 days, photomicrographs were taken. The red line is the margin of unhealed wound that was not re-epithelialized. (B) The size of unhealed wound area was calculated with an image analysis program. The values shown are the means±SEM of 4 mice per group. *p<0.05 vs. collagen gel group; †p<0.05 vs. ASCs group.

• 

  Fig. 3 Adipose-derived stem cells (ASCs) or dermal fibroblasts (DFs) wound scars were thicker than collagen gel alone. (A) At 28 days after surgery, whitish scars of collagen gel (a), ASCs in collagen gel (b) or DFs in collagen gel (c) wounds were harvested and processed for histological examination. (B) The thickness of dermal portion of wound scars was measured with an image analysis program. The values shown are the means±SEM of 4 mice per group (H&E, ×200). *p<0.05 vs. collagen gel group.

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