J Korean Med Sci.  2010 May;25(5):746-751. 10.3346/jkms.2010.25.5.746.

Effects of Adipose-derived Stromal Cells and of their Extract on Wound Healing in a Mouse Model

• Lim JS
• Yoo G

• KMID: 1713960
• DOI: http://doi.org/10.3346/jkms.2010.25.5.746

Abstract

In this study, the authors investigated the effects of adipose-derived stromal cells (ADSCs) and of their extract on wound healing. After creating wound healing splint model on the backs of mice, ADSCs and their extract were applied. Wound healing rates were calculated at 3, 5, 7, 10, and 14 days after the wounding, and tissues were harvested at 7 and 14 days for histological analysis. Wound healing rates were significantly higher at 7, 10, and 14 days in the cell group than in the control, but in the cell extract group wound healing rates were significantly decreased (P<0.05). Histological scores and capillary densities in the cell group were significantly higher at 2 weeks (P<0.05). In the cell group, thick inflammatory cell infiltration and many capillaries were observed at 1 week, and thick epithelium and numerous large capillaries were observed at 2 weeks. The present study suggests that ADSCs accelerate wound healing as known, and the effects of ADSCs on wound healing may be due to replacing insufficient cells by differentiation of ADSCs in the wound and secreting growth factors by differentiated cells, and not due to the effect of factors within ADSCs.

Keyword

Adipose-derived Stromal Cell; Cell Extract; Wound Healing

MeSH Terms

Adipocytes/*transplantation
Animals
Cells, Cultured
*Disease Models, Animal
Humans
Male
Mice
Mice, Inbred BALB C
Stem Cell Transplantation/*methods
Treatment Outcome
Wound Healing/*physiology
Wounds, Penetrating/*pathology/*surgery

Figure

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  Fig. 1 Two 6-mm circular full-thickness skin defects on the back of mouse with silicone rings.

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  Fig. 2 Changes of wound healing rates of the control, the cell, and the cell extract groups at 3, 5, 7, 10, and 14 days after wounding. *P<0.05 compared to control; †P<0.05 compared to the cell group; ‡P<0.05 compared to the cell extract group.

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  Fig. 3 Histological scores of wound in the control, the cell group, and the cell extract groups at 1 and 2 weeks after wounding. *P<0.05 compared to control and the cell extract group.

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  Fig. 4 Micrographs of wound bed and wound margin in each group at 1 and 2 weeks after wounding (H&E stain, 40). Wound edges are indicated by arrows. At 1 week after wounding, the control group shows thin granulation tissue containing many neutrophils and lymphocytes (A), the cell group shows thick inflammatory cell infiltrations with many capillaries (B), and the cell extract group has more granulation tissues than in the control group (C). At 2 weeks after wounding, the control group shows thick granulation tissues with little re-epithelialization (D), the cell group shows slightly thicker epithelium and greater granulation tissue (E), and the cell extract group shows less re-epithelization and granulation tissue than in the cell group at 2 weeks (F).

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  Fig. 5 Micrographs of wound bed and wound margin in each group at 1 and 2 weeks after wounding (Immunohistochemical staining for CD31, ×100). Capillaries with endothelial cells are stained brown (thin arrows). Wound edges are indicated by thick arrows. At 1 week, some small numbers of capillaries are observed in granulation tissues in the control group (A) and the cell extract group (C), but large numbers of capillaries are observed in the cell group (B). At 2 weeks after wounding, increased capillary development is noted in the control group (D), the cell group (E), and the cell extract groups (F). Particularly, in the cell group numerous larger capillaries are observed in comparison with other groups.

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  Fig. 6 Capillary densities in wounds of the control, cell group, and cell extract group at 1 and 2 weeks after wounding. *P<0.05 compared to the control and cell extract groups.

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