Ann Dermatol.  2008 Dec;20(4):184-189. 10.5021/ad.2008.20.4.184.

Expression of Heat Shock Protein 70 in Human Skin Cells as a Photoprotective Function after UV Exposure

• Roh BH
• Kim DH
• Cho MK
• Park YL
• Whang KU

• KMID: 2156390
• DOI: http://doi.org/10.5021/ad.2008.20.4.184

Abstract

BACKGROUND: Human skin is exposed to various environmental stresses, such as heat, cold, and ultraviolet (UV) radiation. Heat shock proteins (HSPs) induced by temperature elevations, as a physiologic response to mediate repair mechanisms and reduce cellular damage.
OBJECTIVE
The purpose of this study was to investigate the induction of HSPs in human skin cells after UV exposure.
METHODS
We performed immunoblotting using a specific monoclonal antibody to the HSP70 family, one of the best-conserved stress proteins in humans, with cultured normal human keratinocytes, A431 cells, human melanocytes, SK30 cells, and human dermal fibroblasts (HDF).
RESULTS
Our results indicated that high expression of HSP70 in the unstressed state was noted in epidermal cells, including normal human keratinocytes, A431 cells, human melanocytes, and SK30 cells, but epidermal cells showed no additional up-regulation of HSP70 after UV irradiation. On the other hand, HDF expressed very small amounts of HSP70 at baseline, but significantly higher amounts of HSP70 after UV exposure.
CONCLUSION
These findings suggest that constitutive expression of HSP70 in epidermal cells may be an important mechanism for protection of the human epidermis from environmental stresses, such as sunlight exposure.

Keyword

Fibroblast; Heat shock protein; Keratinocytes; Melanocytes; UV exposure

MeSH Terms

Cold Temperature
Epidermis
Fibroblasts
Hand
Heat-Shock Proteins
Hot Temperature
HSP70 Heat-Shock Proteins
Humans
Immunoblotting
Keratinocytes
Melanocytes
Skin
Sunlight
Up-Regulation
HSP70 Heat-Shock Proteins
Heat-Shock Proteins

Figure

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  Fig. 1 Western blot analysis of HSP70 expression from keratinocytes. UV exposure did not up-regulate HSP70 expression. Slight up-regulation was noted at 50 J/cm2 after 10% UVB+90% UVA exposure.

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  Fig. 2 Western blot analysis of HSP70 expression from A431 cells. No additional expression of HSP70 occurred after UV radiation compared to baseline. They showed a reverse dose-dependent pattern of HSP70 expression in the UVA range after 10 hours of incubation.

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  Fig. 3 Western blot analysis of HSP70 expression from melanocytes. The irradiation of UVA at doses of 32 and 48 J/cm2 resulted in a decreased expression of HSP70 compared to 4 and 16 J/cm2.

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  Fig. 4 Western blot analysis of HSP70 expression from SK30 cells. The increased expression of HSP70 was noted after heat treatment. HSP70 was not up-regulated by UV exposure.

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  Fig. 5 Western blot analysis of HSP70 expression from dermal fibroblasts. They expressed very high amounts of HSP70 compared to baseline after UVA and 10% UVB+90% UVA exposure.

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