J Korean Soc Vasc Surg.  2001 Nov;17(2):185-191.

Expression of Heat Shock Protein 70 in Vein Endothelial Cells Induced by Shear Stress

Affiliations
  • 1Institute of Advanced Machinery and Design, Seoul National University, Korea.
  • 2Bio Clue & Solution Co., Ltd., Korea.
  • 3Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Korea.
  • 4School of Electrical Engineering, Seoul National University, Seoul, Korea. jkchang@amed.snu.ac.kr

Abstract

PURPOSE: Endothelial cells (ECs) are exposed to continuous shear stress due to the blood flow. Heat shock protein (hsp) 70 is a well-known stress-response protein. The objective of this study is to investigate the expression of hsp70 in human umbilical vein endothelial cells induced by shear stress. METHOD: The pattern of hsp70 was investigated by the in vitro laminar flow system and its image was analyzed by PV-WAVE software program. The image of heat shock-induced hsp70 expression was analyzed and compared with that of shear stress-induced hsp70 expression. RESULT: Under the condition of shear stress produced by flow, the ECs change their morphology and lineup along the flow direction. As to expression of flow stress-induced hsp70, it was accumulated in perinuclear region of endothelial cells, which is different from heat shock-induced hsp70 exression.
CONCLUSION
ECs alignment along the direction of the flow shear stress and changes their phenotype are due to the remodeling of the actin microfilaments. Perinuclear expression of hsp70 may play an important roles in this shear stress induced remodeling response.

Keyword

Heat shock protein; Shear; Stress

MeSH Terms

Actin Cytoskeleton
Endothelial Cells*
Heat-Shock Proteins*
Hot Temperature*
HSP70 Heat-Shock Proteins*
Human Umbilical Vein Endothelial Cells
Phenotype
HSP70 Heat-Shock Proteins
Heat-Shock Proteins
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