Ewha Med J.  1980 Dec;3(4):157-162. 10.12771/emj.1980.3.4.157.

Qualitative Alteration in Hepatic Microsomal Cytochrome P-450 Apoproteins Associated with Bile Duct Ligation, and the Administration of Cholic Acid and Phenobarbital

• Sung NE
• Kim BH
• Hong YS
• Choi KJ

• KMID: 2134970
• DOI: http://doi.org/10.12771/emj.1980.3.4.157

Abstract

We have investigated in rat liver whether different forms of cytochrome P-450 are alterated in hepatic disorders associated with impaired drug metabolism. Total hepatic cytochrome P-450 is decreased after either bile duct ligation or the administration of estradiol. In contrast, phenobarbital administrated alone increase hepatic content of cytochrome P-450, and when administrated with estradiol the reduction in cytochrome P-450 was prevented. Four forms of microsomal cytochrome P-450 apoproteins, ranging in molecular weight from 50 000 to 58,000, were tentatively identified in a sodium dodecyl sulfate(SDS)-6M urea polyaocrylamide gel electrophoresis system. Phenobrbital administration icreased primarily band IV(50,000 daltons). Bile duct ilgation was associated with a marked reduction in bands II and III while bands II and III were decreased with estradiol benzoate administration. Sumultaneous administration of phenobarbital and estradiol demonstrated a return of band I and an increase in density of bands III and IV. Simultaneous administration of cholic acid and estradiol demonstrated a return of band I and not altered in band III and IV. These studies support the hypothesis that multiple forms of cytochrome P-450 are present in liver microsomal membranes and that alterations in spenific apoproteins may be associated with an increase or a decrease in the functional properties of cytochrome P-450.

MeSH Terms

Animals
Apoproteins*
Benzoates
Bile Ducts*
Bile*
Cholic Acid*
Cytochrome P-450 Enzyme System*
Cytochromes*
Electrophoresis
Estradiol
Ligation*
Liver
Membranes
Metabolism
Molecular Weight
Phenobarbital*
Rats
Sodium
Urea
Apoproteins
Benzoates
Cholic Acid
Cytochrome P-450 Enzyme System
Cytochromes
Estradiol
Phenobarbital
Sodium
Urea