Ewha Med J.  1982 Mar;5(1):1-9. 10.12771/emj.1982.5.1.1.

Effect of Hydrocortisone on Cytochrome P-450 Apoproteins in Bile Duct Ligated and Adrenalectomized Rats

• Lee MS

• KMID: 1967857
• DOI: http://doi.org/10.12771/emj.1982.5.1.1

Abstract

This study is concerned with cytochrome P-450 contents and different forms of cytochrome P-450 after administration of hydrocortisone in common bile duct ligated and adrenalectomized rats. Content of total hepatic cytochrome P-450 is decreased after either common bile duct ligation or adrenalectomy. In contrast, hydrocortisone increases content of hepatic cytochrome P-450. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to examine the poly-petide pattern of rat liver endoplasmic reticulum membrane fraction. This report describes the chromatographic separation of four forms of microsomal cytochrome P-450 apoproteins, whose molecular weights ranging from 50,000 to 58,000 indentified in a sodium dodecyl sulfate(SDS)-6M urea-polyacrlamide gel electrophoresis system. Bile duct ligation was associated with a reduction in band I,II,III and IV. And administration of hydrocortisone after bile duct ligation plus adrenalectomy demonstrated a return of band II and III. Hydrocortisone administrated alone increased band III, while band 1 was decreased.

MeSH Terms

Adrenalectomy
Animals
Apoproteins*
Bile Ducts*
Bile*
Common Bile Duct
Cytochrome P-450 Enzyme System*
Cytochromes*
Electrophoresis
Endoplasmic Reticulum
Hydrocortisone*
Ligation
Liver
Membranes
Molecular Weight
Rats*
Sodium
Apoproteins
Cytochrome P-450 Enzyme System
Cytochromes
Hydrocortisone
Sodium