Ewha Med J.  1981 Sep;4(3):83-89. 10.12771/emj.1981.4.3.83.

The Effects of Phenobarbital or Cholic Acid on Hepatic Microsomal Cytochrome P450 and Hydroxylation of 2-AAF in Cholestatic Rats

• Kim BH
• Hong YS
• Sung NE

• KMID: 1969538
• DOI: http://doi.org/10.12771/emj.1981.4.3.83

Abstract

In cholestatic rats, effects of phenobarbital or cholic acid on hepatic microsomal cytochrome p-450 and b5 were investigated. The total contents of both cytochrome p-450 and cytochrome b5 were decreased after bile duct ligation and the administration of estradiol. When cholic acid or phenobarbital was adminstrated in cholestatic rats, the decrease of cytochrome p-450 was prevented. The effects of cholic acid or phenobarbital on both ring-hydroxylation and N-hydroxylation of AAF in cholestatic rat hepatic microsomal fraction were studied. N-hydroxylation of AAF in bile duct ligated rat liver microsomes was reduced by 34%, but ring-hydroxylation was increased by 51%. In estradiol administrated rat, both ring-hydroxylation and N-hydroxylation of AAD was increased by 20 to 25%. In cholic acid administration, both ring-hydroxylation and N-hydroxylation was increased by about 10%. N-hydroxylation of AAF in phenobarbital treated rats was reduced more than ring-hydroxylation was reduced compared to the bile duct ligated group. Estradiol treated group which administrated with cholic acid or phenobarbital exhibited inhibited effects of N-hydroxylation.

MeSH Terms

2-Acetylaminofluorene*
Animals
Bile Ducts
Cholic Acid*
Cytochrome P-450 Enzyme System*
Cytochromes b5
Cytochromes*
Estradiol
Hydroxylation*
Ligation
Microsomes, Liver
Phenobarbital*
Rats*
2-Acetylaminofluorene
Cholic Acid
Cytochrome P-450 Enzyme System
Cytochromes
Cytochromes b5
Estradiol
Phenobarbital