J Korean Soc Magn Reson Med.  2012 Apr;16(1):31-39. 10.13104/jksmrm.2012.16.1.31.

Evaluation of Optimal Combination of Commercially Available Superparamagnetic Iron Oxide Nanoparticles and Transfection Agents for Labelling of Human Mesenchymal Stem Cells

• Kim SH
• Oh SN
• Park YH
• Kang WK
• Ahn KJ
• Chung SK

• KMID: 2099835
• DOI: http://doi.org/10.13104/jksmrm.2012.16.1.31

Abstract

PURPOSE
To determine the optimal combination of commercially available superparamagnetic iron oxide (SPIO) nanoparticles with transfection agents (TA).
MATERIALS AND METHODS
Protamine sulfate (Pro) and poly-L-lysin (PLL) were incubated with ferumoxide and ferucarbotran in human mesenchymal stem cells at various concentrations, and cellular viability were evaluated. Cellular iron uptake was qualitatively and quantitatively evaluated. Cell visibility was assessed via MR imaging and the T2-relaxation time was calculated.
RESULTS
The cellular viabilities with ferucarbotran were more significantly decreased than those with ferumoxide (p < 0.05). Iron uptake with ferumoxide was significantly higher than that for those with with ferucarbotran. The T2-relaxation time was observed to be shorter with ferumoxide in comparison to those with ferucarbotran (p < 0.05). Ferumoxide at a concentration of 25 microg/ml in combination with either Pro or PLL at a concentration of 3.0 microg/ml did not adversely impact cell viability, maximized iron uptake, and exhibited a lower T2-relaxation time in comparison to other combinations.
CONCLUSION
Stem cells with ferumoxide exhibited a higher cellular viability and iron uptake in comparison to ferucarbotran- treated stem cells. A 25 microg/ml of ferumoxide with a 3.0 microg/ml of TA is sufficient to label mesenchymal stem cells.

Keyword

Cell labeling; Contrast media; Experimental studies; Iron; Magnetic resonance (MR)

MeSH Terms

Cell Survival
Contrast Media
Dextrans
Ferric Compounds
Humans
Iron
Magnetite Nanoparticles
Mesenchymal Stromal Cells
Nanoparticles
Protamines
Stem Cells
Transfection
Contrast Media
Dextrans
Ferric Compounds
Iron
Magnetite Nanoparticles
Protamines

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