Korean J Anat.
2005 Oct;38(5):403-411.
Kinesin Superfamily KIF1A Protein Binds to Synaptotagmin XI
- Affiliations
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- 1Department of Biochemistry, College of Medicine, Inje University, Busan, Korea. daehyun@inje.ac.kr
- 2Department of Parasitology, College of Medicine, Inje University, Busan, Korea.
- 3Department of Psychiatry, College of Medicine, Inje University, Busan, Korea.
- 4Department of Neurology, College of Medicine, Inje University, Busan, Korea.
- 5Department of Pathology, College of Medicine, Inje University, Busan, Korea.
- 6Dapartment of Anatomy, College of Medicine, Yeungnam University, Daegu, Korea.
Abstract
- The kinesin proteins (KIFs) make up a large superfamily of molecular motors that transport cargo such as vesicles, protein complexes, and organelles. KIF1A is a monomeric motor that conveys synaptic vesicle precursors and plays an important role in neuronal function. Here, we used the yeast two-hybrid system to identify the neuronal protein (s) that interacts with the tail region of KIF1A and found a specific interaction with synaptotagmin XI. The amino acid residues between 830 and 1300 of KIF1A are required for the interaction with synaptotagmin XI. KIF1A also bound to the tail region of synaptotagmin IV but not to other synaptotagmin in the yeast two-hybrid assay. KIF1A interacted with GST-synaptotagim XI fusion proteins, but not with GST alone. An antibody to synaptotagmin XI specifically co-mmunoprecipitated KIF1A associated with synaptotagimin from mouse brain extracts. These results suggest that KIF1A motor protein transports of synaptotagmin XI-containing synaptic vesicle precursors along microtubule.
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