Korean J Immunol.  1997 Sep;19(3):407-419.

Study on the Production and Function of Macrophage Migration Inhibitory Factor ( MIF ) : Effect of Recombinant MIF and MIF cDNA on the Induction of Cytokine mRNA

• Park JW
• Suh MH
• Suh SI
• Baek WK
• Han IS
• Choe BK
• Ahn SY
• Yoon SJ
• Shin SW
• Bae JH

Abstract

In order to study the functions of migration inhibitory factor (MIF) as macrophage activating cytokine and to investigate the possibility of MIF cDNA as gene therapeutic agent or adjuvant, we produced recombinant MIF (rMIF), anti-MIF antibody and pcDNA I plasmid containing mMIF cDNA (mMIF plasmid). We have investigated the effects of recombinant mMIF or mMIF plasmid on the expression of immune response-related gene in the mouse peritoneal macrophage or splenocyte. Recombinant mMIF produced by Baculovirus expression system was biologically active; it increased mRNA expression of tumor necrosis factor (TNF)-a, Interleukin (IL)-1, IL-6, granulocyte monocyte-colony stimulating factor (GM-CSF), nitric oxide synthase (NOS), Fas and Bcl-x when applied to the cultures of mouse peritoneal macrophage. Anti-mMIF antibody blocked these effects of mMIF on macrophage. Plasmid DNA carrying MIF cDNA inoculated into mouse peritoneal cavity also increased mRNA transcriptions from TNF, IL-1, IL-6, IL-12, GM-CSF, NOS genes of peritoneal macrophage. It enhanced proliferation of splenocyte stimulated with phorbol myristate acetate and IL-2 mRNA expression of splenocytes. Frorn these results, we conclude that rMIF is a strong macrophage activating factor and especially MIF plasmid can be used as an immune potentiating DNA drug in gene therapy for cancer or DNA adjuvant in vaccination in future.

Keyword

MIF; cDNA adjuvant

MeSH Terms

Animals
Baculoviridae
DNA
DNA, Complementary*
Genetic Therapy
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocytes
Interleukin-1
Interleukin-12
Interleukin-2
Interleukin-6
Interleukins
Macrophages*
Macrophages, Peritoneal
Mice
Nitric Oxide Synthase
Peritoneal Cavity
Plasmids
RNA, Messenger*
Tetradecanoylphorbol Acetate
Tumor Necrosis Factor-alpha
Vaccination
DNA
DNA, Complementary
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1
Interleukin-12
Interleukin-2
Interleukin-6
Interleukins
Nitric Oxide Synthase
RNA, Messenger
Tetradecanoylphorbol Acetate
Tumor Necrosis Factor-alpha