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Expression and Purification of Recombinant Mayaro Virus Envelope Glycoproteins E1 and E2 to Develop a Mayaro Virus Detection System

Yi SY, Yoon K, Kwon J, Kim KE, Park K, Shin YB

Mayaro virus (MAYV) is a mosquito-transmitted alphavirus that produces an acute, usually non-fatal, febrile illness including Mayaro fever. Like other alphaviruses, the MAYV E1 and E2 envelope glycoproteins are major...
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Immunogenicity and efficacy of Schmallenberg virus envelope glycoprotein subunit vaccines

Endalew AD, Faburay B, Trujillo J, Gaudreault NN, Davis AS, Shivanna V, Sunwoo SY, Ma W, Drolet BS, McVey DS, Morozov I, Wilson WC, Richt JA

The Schmallenberg virus (SBV) is an orthobunyavirus that causes abortions, stillbirths, and congenital defects in pregnant sheep and cattle. Inactivated or live attenuated vaccines have been developed in endemic countries,...
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Development of a HA1-specific enzyme-linked immunosorbent assay against pandemic influenza virus A H1N1

Shim DH, Kim MJ, Cha HR, Park ES, Kim AR, Park JH, Park HC, Song D, Lee JM

PURPOSE: Enzyme-linked immunosorbent assay (ELISA) has been used in the diverse field to evaluate influenza virus infection; for the surveillance, diagnosis, efficacy evaluation, and development of the vaccine. The aim...
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Vaccine containing G protein fragment and recombinant baculovirus expressing M2 protein induces protective immunity to respiratory syncytial virus

Jo YM, Kim J, Chang J

PURPOSE: Respiratory syncytial virus (RSV) can cause serious respiratory illnesses such as pneumonia, asthma, and bronchiolitis in infants and elderly or immunocompromised individuals. An RSV vaccine has yet to be...
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Expression and serological application of recombinant epitope-repeat protein carrying an immunodominant epitope of Newcastle disease virus nucleoprotein

Gaikwad SS, Lee HJ, Kim JY, Choi KS

PURPOSE: The aim of the present study was to develop a serodiagnostic test for differentiation infected from vaccinated animal (DIVA) strategy accompanying the marker vaccine lacking an immunodominant epitope (IDE)...
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Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus

Kim YH, Lee J, Kim YE, Chong CK, Pinchemel , Reisdörfer F, Coelho JB, Dias RF, Bae PK, Gusmão ZP, Ahn HJ, Nam HW

We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV....
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Indirect ELISA for the Detection of Rabies Virus Antibodies in Dog Sera

Yang DK, Kim HH, Lee SH, Ji M, Cho IS

Rabies is known as the most fatal disease in all warm-blooded animals, including dogs. Among animals that transmit rabies, dogs are mainly responsible for transmitting animal rabies in Asian countries....
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Influenza M1 Virus-Like Particles Consisting of Toxoplasma gondii Rhoptry Protein 4

Lee SH, Lee DH, Piao Y, Moon EK, Quan FS

Toxoplasma gondii infections occur throughout the world, and efforts are needed to develop various vaccine candidates expressing recombinant protein antigens. In this study, influenza matrix protein (M1) virus-like particles (VLPs)...
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Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration

Park YC, Song JM

PURPOSE: Nitrocellulose membrane–based filtration system (NCFS) is widely used for protein concentration. In this study, we applied NCFS for production of virus-like particle (VLP) as a vaccine candidate and evaluated...
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Production and characterization of VP6-specific monoclonal antibodies against bovine group C rotavirus

Kim DU, Jeong SA, Lee SC, Cho KO, Kang SY

Rotaviruses are enteric pathogens causing acute watery dehydrating diarrhea in humans and animals. The importance of group C rotavirus (GpC-RV) infections has not been established as the studies on the...
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Expression of human norovirus VP1 gene and VP1-specific monoclonal antibodies

Kim JW, Kong YJ, Kim MS, Lee HJ, Lee SW, Kang SY

Norovirus (NoV) is an etiologic agent of human and animal acute gastroenteritis and is a member of the family Caliciviridae. NoV is classified based on nucleotide sequences of the VP1...
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Protein Expression of the Bombyx mori Decapentaplegic Gene using the Baculovirus Expression Vector System

Jeong ST, Park SW

The Bombyx mori decapentaplegic gene is one of the conserved genes in vertebrate and invertebrates. The TGF-beta superfamily contains conserved polypeptide growth factors that play important roles in different cellular...
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Biological Property of Recombinant Hemagglutinin-Neuraminidase Protein of Avian Paramyxovirus Type 6 Expressed by Recombinant Baculovirus

Kim JY, Lee HJ, Kye SJ, Kim S, Seul HJ, Kim SE, Lee HS, Jung SC, Choi KS

Hemagglutination inhibition (HI) test employing whole virus antigen is a prescribed serological test for serotyping, diagnosis and surveillance for avian paramyxoviruses (APMVs). For use as alternative to the virus antigen,...
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Development of inactivated Akabane and bovine ephemeral fever vaccine for cattle

Yang DK, Kim HH, Jo HY, Choi SS, Cho IS

Akabane and bovine ephemeral fever (BEF) viruses cause vector-borne diseases. In this study, inactivated Akabane virus (AKAV)+Bovine ephemeral fever virus (BEFV) vaccines with or without recombinant vibrio flagellin (revibFlaB) protein...
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Inactivated genotype 1 Japanese encephalitis vaccine for swine

Yang DK, Nah JJ, Kim HH, Song JY

PURPOSE: Japanese encephalitis is a reproductive disorder caused by Japanese encephalitis virus (JEV) in swine. Recent genotype (G) shift phenomenon (G3 to G1) in the Asia-wide has posed a challenge...
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Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus

Choi KS, Kye SJ, Jeon WJ, Park MJ, Kim S, Seul HJ, Kwon JH

A recombinant hemagglutinin-neuraminidase (rHN) protein from Newcastle disease virus (NDV) with hemagglutination (HA) activity was expressed in Spodoptera frugiperda cells using a baculovirus expression system. The rHN protein extracted from...
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Baculovirus-based Vaccine Displaying Respiratory Syncytial Virus Glycoprotein Induces Protective Immunity against RSV Infection without Vaccine-Enhanced Disease

Kim S, Chang J

BACKGROUND: Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract diseases in infancy and early childhood. Despite its importance as a pathogen, there is no licensed...
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Expression and Purification of Phospholipase C-beta4, and Chimeric Phospholipase C and Characterization of Them

Park DJ

BACKGROUND: Phospholipase C-beta4 (PLC-beta4) is known to be one of the most important signal transducing molecules; however, its biophysical and chemical characteristics are not well known due to the difficulty...
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Protein Expression of the Human Norovirus Capsid Gene using the Baculovirus Expression System

Jin JY, Park CJ, Park SW, Paik SY

Human norovirus (HuNoV) is the major etiological agent of nonbacterial gastroenteritis worldwide. However, due to the absence of a rapid and sensitive diagnostic system, detection and monitoring have been limited....
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Sequence Analysis and Expression of ORF5 in Korean Isolate of Porcine Reproductive and Respiratory Syndrome Virus

Yi SS, Sun JS, Kim HS, Kang SY, Hahn TW

Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of reproductive failure and respiratory disorders in pigs. The viral genome consists of eight overlapping open reading frames (ORFs)....
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