J Bacteriol Virol.  2010 Sep;40(3):123-130. 10.4167/jbv.2010.40.3.123.

Mouse Dual Ig Domain Containing Cell Adhesion Molecule Protein Expression and Purification Using the Baculovirus Expression Vector System

• Park SW
• Choi JH
• Goo TW
• Kim SR
• Lee GG
• Kang SW

• KMID: 2055049
• DOI: http://doi.org/10.4167/jbv.2010.40.3.123

Abstract

A baculovirus expression vector system (BEVS) is used routinely to produce recombinant proteins in the milligram scale. Dual Ig domain containing cell adhesion molecule (DICAM) belongs to the type I class of transmembrane proteins. It consists of a signal peptide, two V-type Ig domains in the extracellular region, and a short cytoplasmic tail of 442 amino acids. To purify the recombinant DICAM protein from cells overexpressing the mouse full-length DICAM gene, recombinant baculovirus is infected and recovered in the Sf9 cells. As a result, mouse DICAM protein was efficiently expressed and extracted from the insect cells using the BEVS. This recombinant protein can be used in further studies for functional test of DICAM protein in the cells.

Keyword

Baculovirus expression vector system; Dual Ig domain containing cell adhesion molecule; Recombinant protein

MeSH Terms

Amino Acids
Animals
Baculoviridae
Cell Adhesion
Cytoplasm
Insects
Mice
Protein Sorting Signals
Proteins
Recombinant Proteins
Sf9 Cells
Amino Acids
Protein Sorting Signals
Proteins
Recombinant Proteins