Immune Netw.  2003 Mar;3(1):8-15. 10.4110/in.2003.3.1.8.

CD30-Mediated Regulation of Cell Adhesion Molecule Expression on Murine T Cells

• Nam SY

• KMID: 2167965
• DOI: http://doi.org/10.4110/in.2003.3.1.8

Abstract

BACKGROUND
CD30 is a member of TNF receptor family and expressed on lymphocytes and other hematopoietic cells following activation as well as Hodgkin and Reed- Sternberg cells in Hodgkin's lymphoma. In this study, CD30-mediated regulation of cell adhesion molecule expression on normal activated mouse T cells was investigated. METHODS: Mouse T cells were activated with anti-CD3 antibody for induction of CD30, which was cross-linked by immobilized anti-CD30 antibody. RESULTS: High level of CD30 expression on T cells was observed on day 5, but only little on day 3 even under culture condition resulting in an identical T cell proliferation, indicating that CD30 expression requires a prolonged stimulation up to 5 days. Cross-linking of CD30 alone altered neither proliferation nor apoptosis of normal activated T cells. Instead, CD30 appeared to promote cell adherence to culture substrate, and considerably upregulated ICAM-1 and, to a lesser extent, ICAM-2 expression on activated T cells, whereas CD2 and CD18 (LFA-1) expression was not affected. None of cytokines known as main regulators of ICAM-1 expression on tissue cells (IL 4, IFNgamma and TNFalpha) enhanced ICAM-1 expression in the absence of CD30 signals. On the other hand, addition of NF-kappaB inhibitor, PDTC (0.1 mM) completely abrogated the CD30-mediated upregulation of ICAM-1 expression, but not CD2 and ICAM-2 expression. CONCLUSION: This results support that CD30 upregulates ICAM-1 expression of T cell and such regulation is not mediated by higher cytokine production but NF-kappaB activation. Therefore, CD30 may play important roles in T-T or T-B cell interaction through regulation of ICAM-1, and -2 expression.

Keyword

CD30; cell adhesion molecules; ICAM-1 (CD54); T cells

MeSH Terms

Animals
Apoptosis
Cell Adhesion Molecules
Cell Adhesion*
Cell Communication
Cell Proliferation
Cytokines
Hand
Hodgkin Disease
Humans
Intercellular Adhesion Molecule-1
Lymphocytes
Mice
NF-kappa B
Receptors, Tumor Necrosis Factor
T-Lymphocytes*
Up-Regulation
Cell Adhesion Molecules
Cytokines
Intercellular Adhesion Molecule-1
NF-kappa B
Receptors, Tumor Necrosis Factor