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Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist

Hong J, Qu P, Wuest TR, Huang H, Huang C, Lin PC

Neutrophilic granule protein (NGP) was previously reported as a granular protein of neutrophils in mouse, but the function has not been known clearly. We found the presence of the possible...
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Transient expression of hemagglutinin antigen from canine influenza virus H3N2 in Nicotiana benthamiana and Lactuca sativa

Maharjan P, Choe S

PURPOSE: Canine influenza virus (CIV), H3N2, carries potentiality for zoonotic transmission and genetic assortment which raises a concern on possible epidemics, and human threats in future. To manage possible threats,...
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A new biomarker for the early diagnosis of ovarian torsion: SCUBE-1

Uyanikoglu H, Hilali NG, Yardimciel M, Koyuncu I

OBJECTIVE: Prompt diagnosis and management are essential for saving the adnexal organs from infarction in cases of ovarian torsion (OT). This study aimed to determine the diagnostic significance of signal...
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Structural analyses of putative porcine cluster of differentiation (CD) 24 gene

Ko H, Chai HH, Jang GW, Park JE, Kim JM, Lee KT

Cluster of differentiation (CD) 24 or heat stable antigen 24 (HSA) molecule is a mucin-type glycoprotein attached to the cell surface by glycosylphosphatidylinositol (GPI)-anchor, promoting adhesive interactions between cells or...
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Structural Analysis of Recombinant Human Preproinsulins by Structure Prediction, Molecular Dynamics, and Protein-Protein Docking

Jung SH, Kim CK, Lee G, Yoon J, Lee M

More effective production of human insulin is important, because insulin is the main medication that is used to treat multiple types of diabetes and because many people are suffering from...
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Characterization of Pv92, a Novel Merozoite Surface Protein of Plasmodium vivax

Lee SK, Wang B, Han JH, Nyunt MH, Muh F, Chootong P, Ha KS, Park WS, Hong SH, Park JH, Han ET

The discovery and understanding of antigenic proteins are essential for development of a vaccine against malaria. In Plasmodium falciparum, Pf92 have been characterized as a merozoite surface protein, and this...
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IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2

Lee MF, Chang CW, Song PP, Hwang GY, Lin SJ, Chen YH

PURPOSE: Cockroaches are the second leading allergen in Taiwan. Sensitization to Per a 2, the major American cockroach allergen, correlates with clinical severity among patients with airway allergy, but there...
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Genome-Wide Identification and Characterization of Novel Laccase Genes in the White-Rot Fungus Flammulina velutipes

Kim HI, Kwon OC, Kong WS, Lee CS, Park YJ

The aim of this study was to identify and characterize new Flammulina velutipes laccases from its whole-genome sequence. Of the 15 putative laccase genes detected in the F. velutipes genome,...
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The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention

Kim DG, Song CS, Choi IS, Park SY, Lee JB, Lee SS

The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained...
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Cloning and Characterization of a Novel Laccase Gene, fvlac7, Based on the Genomic Sequence of Flammulina velutipes

Kim JK, Lim SH, Kang HW

  • KMID: 2312683
  • Mycobiology.
  • 2013 Mar;41(1):37-41.
Laccases (EC 1.10.3.2) are copper-containing polyphenol oxidases found in white-rot fungi. Here, we report the cloning and analysis of the nucleotide sequence of a new laccase gene, fvlac7, based on...
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Molecular characterization of the feline T-cell receptor gamma alternate reading frame protein (TARP) ortholog

Weiss AT, von Deetzen MC, Hecht W, Reinacher , Gruber AD

  • KMID: 2391992
  • J Vet Sci.
  • 2012 Dec;13(4):345-353.
T-cell receptor gamma alternate reading frame protein (TARP) is expressed by human prostate epithelial, prostate cancer, and mammary cancer cells, but is not found in normal mammary tissue. To date,...
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Purification and Characterization of Helicobacter pylori gamma-Glutamyltranspeptidase

Song JY, Choi YJ, Kim JM, Kim YR, Jo JS, Park JS, Park HJ, Song YG, Lee KH, Kang HL, Baik SC, Youn HS, Cho MJ, Rhee KH, Lee WK

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and...
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Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris

Huy ND, Thiyagarajan S, Son YL, Park SM

  • KMID: 2312627
  • Mycobiology.
  • 2011 Jun;39(2):121-124.
The cDNA of endo-1,4-beta-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the alpha-factor signal peptide of Saccharomyces cerevisiae, xylanaseA...
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Mouse Dual Ig Domain Containing Cell Adhesion Molecule Protein Expression and Purification Using the Baculovirus Expression Vector System

Park SW, Choi JH, Goo TW, Kim SR, Lee GG, Kang SW

A baculovirus expression vector system (BEVS) is used routinely to produce recombinant proteins in the milligram scale. Dual Ig domain containing cell adhesion molecule (DICAM) belongs to the type I...
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Nucleolar translocalization of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells

Ahn HJ, Kim S, Nam HW

Toxoplasma gondii GRA10 expressed as a GFP-GRA10 fusion protein in HeLa cells moved to the nucleoli within the nucleus rapidly and entirely. GRA10 was concentrated specifically in the dense fibrillar...
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The Purification and Immunogenicity of TB-14 Recombinant Protein of Mycobacterium tuberculosis

Song HY, Kim YH, Kim CH, Min YK, Kim DJ, Ko KK

BACKGROUND: Culture filtrate proteins secreted by mycobacteria are thought to play an important role in inducing protective immunity and to develop new methods for diagnosing tuberculosis. METHODS: A culture filtrate protein...
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IL-2-enhanced NK Cell Cytotoxicity is Regulated by Adiponectin from Hypothalamo-pituitary-adrenal Axis

Kim K, Yang Y

BACKGROUND: The Hypothalamo-Pituitary-Adrenal (HPA) axis is an important regulator for the body's stress response. As a primary stress responsive system, HPA-axis secretes various neurotransmitters, hormones, and cytokines, which regulates the...
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A Case of 2-Methylbutyryl-CoA Dehydrogenase Deficiency

Lee MS, Park HJ, Lee IK, Choeh K, Yoon HR

  • KMID: 2140985
  • Korean J Pediatr.
  • 2004 Jan;47(1):100-105.
We report a one-day-old Korean boy with 2-Methylbutyryl-CoA dehydrogenase(2-MBCDase) deficiency detected by urine organic acid and acylglycine analysis, plasma acylcarnitine analysis and confirmed by enzyme assay and Western blot. The...
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Intracellular trafficking and metabolic turnover of yeast prepro-alpha-factor-SRIF precursors in GH3 cells

Lee MA, Cheong KH, Shields D, Park SD, Hong SH

  • KMID: 931879
  • Exp Mol Med.
  • 2002 Sep;34(4):285-293.
Chimeric genes coding for prepro region of yeast alpha-factor and anglerfish SRIF were expressed in rat GH3 cells to determine whether yeast signals could regulate hormone processing in mammalian...
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A Molecular Biological Study On The Expression Pattern And Functional Protein Structures Of Proline-Rich Proteins In Human Salivary Glands

Joo JY, Lee SK, Park YW

  • KMID: 1961171
  • J Korean Assoc Oral Maxillofac Surg.
  • 2002 Feb;28(1):31-41.
Proline-rich proteins (PRPs) are major components of human saliva. In order to know the biological roles of PRPs, we explored the expression pattern and functional protein structures of PRPs by...
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