J Korean Neurosurg Soc.  1996 Jul;25(7):1352-1363.

Serotonin 1A Receptor Agonist(8 OH DPAT) Reduces Tissue Lesion Volume in the Rat Middle Cerebral Artery Occlusion Model of Ischemia

• Yoon DH
• Kim YS
• Young W

Abstract

We studied the effectiveness of serotonin 1a agonist(8 OH DPAT) on the 24 hour ionic lesion volume produced by permanent occlusion of the middle cerebral artery(MCAo) in rats. A 4-hour intravenous infusion of 30 micro mg/kg/hr of 8 OH DPAT were given starting at 10 minutes after occlusion. Tissue concentrations of Na, K, and water at infarct and peri-infarct zone were measured by atomic absorption spectroscopy and by wet-dry weight measurements 24 hours after occlusion. Compared with vehicle treatment, 8 OH DPAT treatment reduced tissue water accumulation by 10% and 55% in the frontopyriform cortex(L1) and frontoparietal cortex(L2), sodium accumulation by 20% at L1 and 47% at L2, potassium loss by 24% at L1 and 44% at L2, cell volume fraction loss by 24% at L1 and 47% at L2. Finally the treatment reduced overall lesion volume by about 37%. All these changes were statistically significant at p<0.05. Our findings suggest strongly that 8 OH DPAT is neuroprotective in the rat MCAo model of focal cerebral ischemia.

Keyword

Middle cerebral artery occlusion; Serotonin; 8 OH DPAT; Brain ischemia; Lesion volume

MeSH Terms

Absorption
Animals
Brain Ischemia
Cell Size
Infarction, Middle Cerebral Artery*
Infusions, Intravenous
Ischemia*
Middle Cerebral Artery*
Potassium
Rats*
Receptor, Serotonin, 5-HT1A*
Serotonin*
Sodium
Spectrum Analysis
Potassium
Receptor, Serotonin, 5-HT1A
Serotonin
Sodium