J Korean Soc Transplant.
2006 Jun;20(1):41-48.
Alpha-Melanocyte Stimulating Hormone Protects Pancreatic Islet Dysfunction by Peripheral Blood Mononuclear Cells in vitro
- Affiliations
-
- 1Department of Surgery, Ulsan University College of Medicine & Asan Medical Center, Korea. drksc@amc.seoul.kr
- 2Asan Institute for Life Science, Seoul, Korea.
Abstract
-
PURPOSE: The alpha-melanocyte-stimulating hormone (alpha-MSH) has been shown to interact with various cells of the immune and inflammatory system and down-regulate either the production or the action of the pro-inflammatory cytokines. In this study, we investigated the potential of alpha-MSH on preventing pancreatic islet cell from death and dysfunction by inflammatory cytokines released from peripheral blood mononuclear cells (PBMCs) in rat.
METHODS
Rat pancreatic islets were co-cultured with PBMCs, stimulated by phorbol myrstic acid and ionomycin. alpha-MSH was treated to PBMCs for 2 hours before co-culture. Viability and apoptosis of islets were observed by MTT and FACS. Inflammatory cytokines and nitric oxide (NO) were measured. Insulin release from islet co-cultured with mononuclear cells was checked for the islet function.
RESULTS
In comparison to control group, viability of islets with alpha-MSH treated mononuclear cells was increased and apoptosis was reduced significantly. Inflammatory cytokines such as TNF-alpha and IL-1beta were reduced in alpha-MSH-treated group. NO production in alpha-MSH-treated group was decreased. Insulin secretory function of islet was recovered in condition of alpha-MSH treatment.
CONCLUSION
This study demonstrates that alpha-MSH protects cell death and preserves the secretory function of pancreatic islet cells from the pro-inflammatory reaction of mononuclear cells, and may have the potential to improve the graft survival in clinical islet transplantation.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Immunohistochemical Study on the Distribution of Carbonic Anhydrase Isozymes in Pancreatic Islet of Rat
- The Effect of alpha-MSH on the Morphologic Changes, Survival, and Melanization of Cultured Human Melanocytes
- The Expression of the alpha-Melanocyte Stimulating Hormone (alpha-MSH) and Melanocortin-1 Receptor (MC1R) in the Epidermis of the Vitiligo
- Expression of signal transducing G proteins in human melanoma cell lines
- Generation of Mature Dendritic Cells from Peripheral Blood
