Nutr Res Pract.  2013 Oct;7(5):366-372. 10.4162/nrp.2013.7.5.366.

Effect of green tea extract microencapsulation on hypertriglyceridemia and cardiovascular tissues in high fructose-fed rats

• Jung MH
• Seong PN
• Kim MH
• Myong NH
• Chang MJ

• KMID: 1841307
• DOI: http://doi.org/10.4162/nrp.2013.7.5.366

Abstract

The application of polyphenols has attracted great interest in the field of functional foods and nutraceuticals due to their potential health benefits in humans. However, the effectiveness of polyphenols depends on their bioactivity and bioavailability. In the present study, the bioactive component from green tea extract (GTE) was administrated orally (50 mg/kg body weight/day) as free or in a microencapsulated form with maltodextrin in rats fed a high fructose diet. High fructose diet induced features of metabolic syndrome including hypertriglyceridemia, hyperuricemia, increased serum total cholesterol, and retroperitoneal obesity. In addition, myocardial fibrosis was increased. In rats receiving high fructose diet, the lowering of blood triglycerides, total cholesterol, non esterified fatty acid (NEFA) and uric acid, as well as the reduction in final body weight and retroperitoneal fat weight associated with the administration of GTE, led to a reversal of the features of metabolic syndrome (P < 0.05). In particular, the administration of microencapsulated GTE decreased myocardial fibrosis and increased liver catalase activity consistent with a further alleviation of serum NEFA, and hyperuricemia compared to administration of GTE. Taken together, our results suggest that microencapsulation of the bioactive components of GTE might have a protective effect on cardiovasucular system by attenuating the adverse features of myocardial fibrosis, decreasing uric acid levels and increasing hepatic catalase activity effectively by protecting their bioactivities.

Keyword

Microencapsulation; green tea extract; hypertriglyceridemia; myocardial fibrosis

MeSH Terms

Animals
Biological Availability
Body Weight
Catalase
Cholesterol
Diet
Dietary Supplements
Drug Compounding
Fatty Acids, Nonesterified
Fibrosis
Fructose
Functional Food
Humans
Hypertriglyceridemia
Hyperuricemia
Insurance Benefits
Intra-Abdominal Fat
Liver
Obesity
Polyphenols
Polysaccharides
Rats
Tea
Triglycerides
Uric Acid
Catalase
Cholesterol
Fatty Acids, Nonesterified
Fructose
Polyphenols
Polysaccharides
Tea
Triglycerides
Uric Acid

Figure

• 

  Fig. 1 The changes of postprandial serum triglyceride concentrations in experimental groups. NCON: normal diet control, HCON: 65% fructose diet control, HGTE: 65% fructose diet +50 mg/kg bw green tea extract, HMGT: 65% fructose diet +50 mg/kg bw microencapsulated green tea extract. Different letters indicate significantly different values assessed by Duncan's multiple test (P < 0.05).

• 

  Fig. 2 Histological images of myocardial fibrosis. A, B, C, and D: Masson-Trichrome stained sections of myocardium. Images captured at 400 × magnification. A: normal control rat, B: high fructose control rat, C: high fructose plus green tea extract, D: high fructose plus microencapsulated green tea extract.

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