Yonsei Med J.  2013 Nov;54(6):1342-1352. 10.3349/ymj.2013.54.6.1342.

Gastric Autoantigenic Proteins in Helicobacter Pylori Infection

• Park JS
• Lee SJ
• Kim TH
• Yeom J
• Park ES
• Seo JH
• Jun JS
• Lim JY
• Park CH
• Woo HO
• Youn HS
• Ko GH
• Kang HL
• Baik SC
• Lee WK
• Cho MJ
• Rhee KH

• KMID: 1798129
• DOI: http://doi.org/10.3349/ymj.2013.54.6.1342

Abstract

PURPOSE
This study tried to identify novel gastric autoimmune antigens that might be involved in aggravating the atrophic gastritis among patients with Helicobacter pylori infection using two-dimensional immunoblotting analysis.
MATERIALS AND METHODS
Proteins from gastric mucosal antrectomy specimens and AGS cells (gastric adenocarcinoma cell lines derived from a Caucasian patient who had received no prior therapy) were 2-dimensionally immunoblotted separately with a pool of 300 sera from H. pylroi-infected patients at Gyeongsang National University Hospital.
RESULTS
Thirty-eight autoantigenic proteins including alcohol dehydrogenase [NADP+], alpha enolase, gastrokine-1, gastric triacylglycerol lipase, heat shock 70 kDa protein 1, and peroxiredoxin-2 were identified in the gastric mucosal tissue. Fourteen autoantigenic proteins including programmed cell death 6-interacting protein, serum albumin and T-complex protein 1 subunit gamma were identified in the AGS cells. Albumin, alpha-enolase, annexin A3, cytoplasmic actin 1, heat shock cognate 71 kDa protein and leukocyte elastase inhibitor were commonly observed autoantigenic proteins in both gastric mucosal tissue and AGS cells. Alpha-enolase, glutathione S-transferase P, heat shock cognate 71 kDa protein, heat shock 70 kDa protein 1, human mitochondrial adenosine triphosphate synthase (ATP) subunit beta, mitochondrial 60 kDa heat shock protein, peroxiredoxin-2, 78 kDa glucose-regulated protein precursor, tyrosine-protein phosphatase non-receptor type 11 and Tryptophan-Aspartic acid (WD) repeat-containing protein 1 showed 60% or higher amino acid positivity.
CONCLUSION
These newly identified gastric autoimmune antigens might be useful in the control and prevention of gastroduodenal disorders, and might be valuable in breaking the vicious circle that exists in gastroduodenal disorders if their pathophysiological roles could be understood in the progress of chronic atrophic gastritis, gastroduodenal ulcers, intestinal metaplasia, and gastric carcinogenesis.

Keyword

Helicobacter pylori; gastric atrophy; autoantigen; 2D immunoblotting

MeSH Terms

Alcohol Dehydrogenase/metabolism
Autoantigens/*metabolism
Electrophoresis, Gel, Two-Dimensional
Gastric Mucosa/metabolism/microbiology
Helicobacter Infections/*metabolism
Humans
Peptide Hormones/metabolism
Phosphopyruvate Hydratase/metabolism
Alcohol Dehydrogenase
Autoantigens
Peptide Hormones
Phosphopyruvate Hydratase

Figure

• 

  Fig. 1 Autoantigenic proteins visualized by 2-D immunoblotting derived from human gastric mucosa. (A) 2-DE of total protein extracts from human gastric mucosal tissue. (B) 2-D immunoblot of human gastric mucosal tissue by a pool of 300 sera obtained from Helicobacter pylori-seropositive patients. 2-DE, two-dimensional-PAGE.

• 

  Fig. 2 Autoantigenic proteins visualized by 2-D immunoblotting derived from AGS cells. (A) 2-DE of total protein extracts from AGS cells. (B) 2-D immunoblot of total proteins from AGS cells by a pool of 300 sera obtained from Helicobacter pylori-seropositive patients. 2-DE, two-dimensional-PAGE; AGS, gastric adenocarcinoma cell lines derived from a Caucasian patient who had received no prior therapy.

• 

  Fig. 3 Common autoantigenic proteins are indicated with red circles on 2-DE silver staining and immunoblotting of gastric mucosal tissue (A) and AGS cells (B). 2-DE, two-dimensional-PAGE.

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