J Korean Med Sci.  2006 Jun;21(3):500-505. 10.3346/jkms.2006.21.3.500.

Low Dose Radiation Overcomes Diabetes-induced Suppression of Hippocampal Neuronal Cell Proliferation in Rats

• Kang JO
• Kim SK
• Hong SE
• Lee TH
• Kim CJ

• KMID: 1778436
• DOI: http://doi.org/10.3346/jkms.2006.21.3.500

Abstract

We investigated the effect of low dose radiation on diabetes induced suppression of neurogenesis in the hippocampal dentate gyrus of rat. After 0.01 Gy, 0.1 Gy, 1 Gy and 10 Gy radiation was delivered, the dentate gyrus of hippocampus of streptozotocin (STZ)-induced diabetic rats were evaluated using immunohistochemistry for 5-bromo-2-deoxyuridine (BrdU), caspase-3, and terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) staining. The number of BrdU positive cells in the non-diabetic rats, diabetic rats without radiation, diabetic rats with 0.01 Gy radiation, diabetic rats with 0.1 Gy radiation, diabetic rats with 1 Gy radiation and diabetic rats with 10 Gy radiation were 55.4+/-8.5/mm2, 33.3+/-6.4/mm2, 67.7+/-10.5/mm2, 66.6+/-10.0/mm2, 23.5+/-6.3/mm2 and 14.3+/-7.2/mm2, respectively. The number of caspase-3 positive cells was 132.6+/-37.4/mm2, 378.6+/-99.1/mm2, 15.0+/-2.8/mm2, 57.1+/-16.9/mm2, 191.8+/-44.8/mm2 and 450.4+/-58.3/mm2, respectively. The number of TUNEL-positive cells was 24.5+/-2.0/mm2, 21.7+/-4.0/mm2, 20.4+/-2.0/mm2, 18.96+/-2.1/mm2, 58.3+/-7.9/mm2, and 106.0+/-9.8/mm2, respectively. These results suggest low doses of radiation paradoxically improved diabetes induced neuronal cell suppression in the hippocampal dentate gyrus of rat.

Keyword

Radiation; Hippocampus; Diabetes Mellitus

MeSH Terms

Rats, Sprague-Dawley
Rats
Radiotherapy/methods
Neurons/*metabolism
Male
In Situ Nick-End Labeling
Hippocampus/*cytology/metabolism/radiation effects
Diabetes Mellitus, Experimental/radiotherapy
Dentate Gyrus/drug effects/*radiation effects
Cell Proliferation
Caspase 3/metabolism
Bromodeoxyuridine/pharmacology
Apoptosis
Animals

Figure

• 

  Fig. 1 The effect of radiation on the number of 5-bromo-2'-deoxyuridine (BrdU)-positive cells in the dentate gyrus of normal rats. Upper: Photomicrographs of BrdU-positive cells in the dentate gyrus. (A) Control group, (B) 1 Gy radiation group. A scale bar represents 50 µm. Lower: The number of BrdU-positive cells in the dentate gyrus in each group. (A) Control group, (B) 0.1 Gy radiation group, (C) 1 Gy radiation group.

• 

  Fig. 2 The effect of radiation on the number of 5-bromo-2'-deoxyuridine (BrdU)-positive cells in the dentate gyrus of streptozotocin (STZ)-induced diabetic rats. Upper: Photomicrographs of BrdU-positive cells in the dentate gyrus. (A) Control group, (B) streptozotocin (STZ)-induced diabetes group, (C) STZ-induced diabetes and 0.01 Gy radiation group, (D) STZ-induced diabetes and 10 Gy radiation group. A scale bar represents 50 µm. Lower: The number of BrdU-positive cells in the dentate gyrus in each group. (A) Control group, (B) STZ-induced diabetes group, (C) STZ-induced diabetes and 0.01 Gy radiation group, (D) STZ-induced diabetes and 0.1 Gy radiation group, (E) STZ-induced diabetes and 1 Gy radiation group, (F) STZ-induced diabetes and 10 Gy radiation group.

• 

  Fig. 3 The effect of radiation on the number of 5-bromo-2'-deoxyuridine (BrdU)-positive cells in the dentate gyrus of streptozotocin (STZ)-induced diabetic rats. Upper: Photomicrographs of BrdU-positive cells in the dentate gyrus. (A) Control group, (B) streptozotocin (STZ)-induced diabetes group, (C) STZ-induced diabetes and 0.01 Gy radiation group, (D) STZ-induced diabetes and 10 Gy radiation group. A scale bar represents 50 µm. Lower: The number of BrdU-positive cells in the dentate gyrus in each group. (A) Control group, (B) STZ-induced diabetes group, (C) STZ-induced diabetes and 0.01 Gy radiation group, (D) STZ-induced diabetes and 0.1 Gy radiation group, (E) STZ-induced diabetes and 1 Gy radiation group, (F) STZ-induced diabetes and 10 Gy radiation group.

• 

  Fig. 4 The effect of radiation on the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive cells in the dentate gyrus of streptozotocin (STZ)-induced diabetic rats. Upper: Photomicrographs of TUNEL-positive cells in the dentate gyrus. (A) Control group, (B) streptozotocin (STZ)-induced diabetes group, (C) STZ-induced diabetes and 0.01 Gy radiation group, (D) STZ-induced diabetes and 10 Gy radiation group. A scale bar represents 50 µm. Lower: The number of TUNEL-positive cells in the dentate gyrus in each group. (A) Control group, (B) STZ-induced diabetes group, (C) STZ-induced diabetes and 0.01 Gy radiation group, (D) STZ-induced diabetes and 0.1 Gy radiation group, (E) STZ-induced diabetes and 1 Gy radiation group, (F) STZ-induced diabetes and 10 Gy radiation group.

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