Korean J Anesthesiol.  2010 Aug;59(2):104-110. 10.4097/kjae.2010.59.2.104.

Antioxidant effect of lidocaine and procaine on reactive oxygen species-induced endothelial dysfunction in the rabbit abdominal aorta

• Lee JM
• Suh JK
• Jeong JS
• Cho SY
• Kim DW

• KMID: 1766371
• DOI: http://doi.org/10.4097/kjae.2010.59.2.104

Abstract

BACKGROUND
Reactive oxygen species (ROS) induce lipid peroxidation and tissue damage in the endothelium. We tested the antioxidant effect of lidocaine and procaine on ROS-induced endothelial damage in the rabbit aorta.
METHODS
Aortic rings isolated from rabbits were suspended in an organ bath filled with Krebs-Henseleit (K-H) solution bubbled with 5% CO2 and 95% O2 at 37.5degrees C. After precontraction with phenylephrine (PE, 10(-6) M), changes in tension were recorded following a cumulative administration of acetylcholine (ACh 3 x 10(-8) to 10(-6) M). Differences were measured as percentages of ACh-induced relaxation of aortic rings before and after exposure to ROS as generated by electrolysis of the K-H solution. The aortic rings were pretreated with lidocaine or procaine (10(-5) M to 3 x 10(-3) M) to compare their effects, as well as ROS scavengers, catalase, mannitol, sodium salicylate, and deferoxamine, and a catalase inhibitor, 3-amino-1,2,4-triazole (3AT).
RESULTS
Lidocaine and procaine dose-dependently maintained endothelium-dependent relaxation induced by ACh despite ROS activity (P < 0.05 vs control value). The 3AT pretreated procaine (3 x 10(-3) M) group decreased more significantly than the un-pretreated procaine group (P < 0.05).
CONCLUSIONS
These findings suggest that lidocaine and procaine dose-dependently preserve endothelium-dependent vasorelaxation against ROS attack, potentially via hydrogen peroxide scavenging.

Keyword

Catalase; Endothelium; Lidocaine; Procaine; Reactive oxygen species; 3-amino-1,2,4-triazole

MeSH Terms

Acetylcholine
Amitrole
Antioxidants
Aorta
Aorta, Abdominal
Baths
Catalase
Deferoxamine
Electrolysis
Endothelium
Hydrogen Peroxide
Lidocaine
Lipid Peroxidation
Mannitol
Oxygen
Phenylephrine
Procaine
Rabbits
Reactive Oxygen Species
Relaxation
Sodium Salicylate
Vasodilation
Acetylcholine
Amitrole
Antioxidants
Catalase
Deferoxamine
Hydrogen Peroxide
Lidocaine
Mannitol
Oxygen
Phenylephrine
Procaine
Reactive Oxygen Species
Sodium Salicylate