Korean J Anesthesiol.  2013 Jan;64(1):54-60. 10.4097/kjae.2013.64.1.54.

Antioxidant effects of methylprednisolone and hydrocortisone on the impairment of endothelium dependent relaxation induced by reactive oxygen species in rabbit abdominal aorta

• Lee HJ
• Suh JK
• Song HH
• Jeong MA
• Yeom JH
• Kim DW

• KMID: 2236235
• DOI: http://doi.org/10.4097/kjae.2013.64.1.54

Abstract

BACKGROUND
The reperfusion following ischemia produces reactive oxygen species (ROS). We studied the influences of methylprednisolone (MPD) and hydrocortisone (CRT) on ROS effects using the endothelium of rabbit abdominal aorta.
METHODS
Isolated rabbit aortic rings were suspended in an organ bath filled with Krebs-Henseleit (K-H) solution. After precontraction with norepinephrine, changes in arterial tension were recorded following the cumulative administration of acetylcholine (ACh). The percentages of ACh-induced relaxation of aortic rings before and after exposure to ROS, generated by electrolysis of K-H solution, were used as the control and experimental values, respectively. The aortic rings were pretreated with MPD or CRT at the same concentrations, and the effects of these agents were compared with the effects of ROS scavenger inhibitors: superoxide dismutase inhibitor, diethylthiocarbamate (DETCA), and the catalase inhibitor, 3-amino-1,2,4-triazole (3AT).
RESULTS
Both MPD and CRT maintained endothelium-dependent relaxation induced by ACh in a dose-related manner in spite of ROS attack. The restored ACh-induced relaxation of MPD and CRT group was not attenuated by pretreatment of 3AT and DETCA.
CONCLUSIONS
MPD and CRT preserve the endothelium-dependent vasorelaxation against the attack of ROS, in a dose-related manner. Endothelial protection mechanisms of MPD and CRT may be not associated with hydrogen peroxide and superoxide scavenging.

Keyword

Diethylthiocarbamate; Endothelium; Hydrocortisone; Methylprednisolone; Reactive oxygen species; 3-amino-1,2,4-triazole

MeSH Terms

Acetylcholine
Amitrole
Antioxidants
Aorta, Abdominal
Arterial Pressure
Baths
Catalase
Electrolysis
Endothelium
Hydrocortisone
Hydrogen Peroxide
Ischemia
Methylprednisolone
Norepinephrine
Reactive Oxygen Species
Relaxation
Reperfusion
Superoxide Dismutase
Superoxides
Vasodilation
Acetylcholine
Amitrole
Antioxidants
Catalase
Hydrocortisone
Hydrogen Peroxide
Methylprednisolone
Norepinephrine
Reactive Oxygen Species
Superoxide Dismutase
Superoxides