Yonsei Med J.  1992 Mar;33(1):8-13. 10.3349/ymj.1992.33.1.8.

Effects of Na+, K(+)-pump inhibitors on acetylcholine-induced relaxation in the rabbit aorta

• Lee YH
• Ahn DS
• Song HJ
• Kim YH
• Kim HS
• Ahn SH
• Kang BS

• KMID: 1117759
• DOI: http://doi.org/10.3349/ymj.1992.33.1.8

Abstract

The purpose of this study was to investigate the effects of inhibitors of the Na+, K(+)-pump and membrane depolarizing agents on endothelium-dependent acetylcholine-induced relaxation in the rabbit thoracic aorta. Aortic rings were prepared from the rabbit descending thoracic aorta and the contractility of the ring was measured in various conditions such as application of ouabain, exposure to K(+)-free Krebs-Henseleit solution and high K+. Ouabain or exposure to K(+)-free Krebs-Henseleit solution inhibited acetylcholine or sodium nitroprusside-induced relaxation. KCl also inhibited the acetylcholine or sodium nitroprusside-induced relaxation. These results suggest that the Na+, K(+)-pump may play a role in endothelium-dependent acetylcholine-induced relaxation.

Keyword

vascular smooth muscle; endothelium-dependent relaxation; Na+; K+-pump

MeSH Terms

Acetylcholine/*pharmacology
Animal
Aorta/drug effects/physiology
Endothelium, Vascular/physiology
In Vitro
Na(+)-K(+)-Exchanging ATPase/drug effects/*physiology
Nitroprusside/pharmacology
Ouabain/pharmacology
Rabbits
Support, Non-U.S. Gov't
Vasodilation/*drug effects