Korean J Occup Environ Med.  1999 Sep;11(3):340-349.

The Toxicologic effect of TAFMAG on RAW 264.7 cell

• Chang HS
• Kim KA
• Kim EK
• Han JK
• Kim JH
• Kim HW
• Lim Y

Abstract

OBJECTIVES
This study was designed to evaluate cytotoxicity of TAFMAG, which is a trade name of natural mineral fiber mined and produced in China.
METHODS
The cytotoxicity of TAFMAG was evaluated by measuring iron content, lipid peroxidation, erythrocyte hemolysis, and cytotoxicity in vitro. These results were compared with the data of chrystotile and wollastonite as a positive and negative control, respectively.
RESULTS
There was significant increase of Fenton activity in TAFMAG and chrysotile with dose-response pattern. The iron chelating agent, desferrioxamine, significantly decreased Fenton activity of the particulates except wollastonite. TAFMAG and chrysotile fibers significantly increased malondialdehyde concentration from lipid peroxidation of the red blood cell membrane. In erythrocyte hemolysis test, TAFMAG & chrysotile had stronger effect on erythrocyte hemolysis than wollastonite with the concentration of 1,000g/ml. Furthermore, TAFMAG was more hemolytic than chrysotile with the concentration of 5.000 g/ml. There was a significant cytotoxic effect in TAFMAG and chrysotile on RAW cell compared with wollastonite.
CONCLUSIONS
In vitro study suggested that TAFMAG may have a similar health hazard as usual asbestos.

MeSH Terms

Asbestos
Asbestos, Serpentine
China
Deferoxamine
Erythrocytes
Hemolysis
Iron
Lipid Peroxidation
Malondialdehyde
Membranes
Mineral Fibers
Asbestos
Asbestos, Serpentine
Deferoxamine
Iron
Malondialdehyde
Mineral Fibers