Immune Netw.  2004 Dec;4(4):224-228. 10.4110/in.2004.4.4.224.

IFN-gamma Regulates Expression of BRG1 Associated Factor 155/170 and Sensitivity to Steroid in Astrocytes

• Lim JH
• Lee J
• Park JY
• Choi IH

• KMID: 1515489
• DOI: http://doi.org/10.4110/in.2004.4.4.224

Abstract

BACKGROUND
The expression of BRG1 associated factors (BAF) 155 and BAF 170 in response to IFN-gamma or TNF-alpha was studied in astrocytoma cell lines and primary astrocytes. BAFs are complexed with BRG1 and are also associated with activated glucocorticoid for glucocorticoid trans-activation. METHODS: IFN-gamma was pretreated for 18 hrs and cells were incubated with IL-1 or TNF-alpha for 72 hrs or 96 hrs with different concentrations of steroid. Cell death was measured by LDH assay. BAF expression was assayed by RT-PCR. RESULTS: IFN-gamma increased cell death by dexamethasone in LN215 cells but not in LN319 cells. The IFN-gamma increased the expression of BAF 155 and BAF 170 in adult astrocytes and LN215 cells, but IFN-gamma decreased the expression of BAF 155/170 in LN319 cells. The effect of IFN-gamma on the expression of BAF was not as clear in fetal astrocytes as it was in adult astrocytes. CONCLUSION: Our results suggest cytokines produced during immune reaction or immunotherapy may modulate steroid susceptibility of astrocytes and astrocytoma cells by influencing the expression of BAFs.

Keyword

Astrocyte; steroid; BAF155/170; IFN-gamma

MeSH Terms

Adult
Astrocytes*
Astrocytoma
Cell Death
Cell Line
Cytokines
Dexamethasone
Humans
Immunotherapy
Interleukin-1
Tumor Necrosis Factor-alpha
Cytokines
Dexamethasone
Interleukin-1
Tumor Necrosis Factor-alpha