J Bacteriol Virol.  2007 Dec;37(4):241-248. 10.4167/jbv.2007.37.4.241.

The Expression of TLR3 and Cytokines Induced by Poly I:C in Human Retinal Pigment Epithelial Cells

Affiliations
  • 1Department of Microbiology, Institute of Basic Medical Science, Yonsei University, Wonju College of Medicine, Il San Dong 162, Republic of Korea. joopark@yonsei.ac.kr
  • 2Woori Eye Clinic, Joongang Dong, Wonju, Gangwon-do, 220-701, Republic of Korea.

Abstract

In this study, we examined the expression of Toll-like receptor3 (TLR3) by human retinal pigment epithelial cells (RPE) and determined whether exposure to the TLR3 agonist polyinosinic-polycytidylic acid (poly I:C) would induced the expression of cytokines in these cells. RT-PCR revealed that TLR3 was constitutively expressed in human RPE, and its expression was increased by treatment with poly I:C. After treatment with poly I:C, we determined the expression levels of pro-inflammatory cytokines in human RPE using RT-PCR and ELISA. We demonstrated that poly I:C treatment increased the production of TNF-alpha, IL-6, and IL-8 in human RPE. Upon exposure to poly I:C, human RPE initiated antiviral response resulting in the induction of IFN-beta mRNA expression and 2',5'-oligoadenylate synthetase mRNA expression. These results suggest that human RPE may participate in ocular defense mechanism against viral infection through TLR3.

Keyword

Toll-like receptor3; Poly I:C; Viral infection; Retinal pigment epithelium; Interferon

MeSH Terms

2',5'-Oligoadenylate Synthetase
Cytokines*
Enzyme-Linked Immunosorbent Assay
Epithelial Cells*
Humans*
Interferons
Interleukin-6
Interleukin-8
Poly I-C
Retinal Pigment Epithelium
Retinaldehyde*
RNA, Messenger
Tumor Necrosis Factor-alpha
2',5'-Oligoadenylate Synthetase
Cytokines
Interferons
Interleukin-6
Interleukin-8
Poly I-C
RNA, Messenger
Retinaldehyde
Tumor Necrosis Factor-alpha
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