J Bacteriol Virol.  2007 Dec;37(4):213-224. 10.4167/jbv.2007.37.4.213.

Comparison of Proteome Component of Helicobacter pylori in Different Atmospheric CO2 Concentration

Affiliations
  • 1Department of Microbiology, Gyeonsang National University, Jinju, Gyeong-nam 660-751, Republic of Korea. kangssi@gnu.kr
  • 2Department of Pediatrics, Gyeonsang National University, Jinju, Gyeong-nam 660-751, Republic of Korea.
  • 3Department of Gyeongsang Institute of Health Science, Gyeonsang National University, Jinju, Gyeong-nam 660-751, Republic of Korea.
  • 4Research Institute of Life Science, Gyeonsang National University, Jinju, Gyeong-nam 660-701, Republic of Korea.
  • 5Central Laboratory, Gyeonsang National University, Jinju, Gyeong-nam 660-701, Republic of Korea.

Abstract

Helicobacter pylori is a spiral, slow growing gram-negative microaerophilic bacterium. It has been shown to be the etiological agent of gastroduodenal diseases, such as chronic gastritis, gastric and duodenal ulcers, and gastric cancer. General culture condition of H. pylori is 5% O2, 10% CO2 and 100% humid atmosphere. We have compared proliferation protein expression profile of H. pylori incubated under normal microaerophilic (10% CO2) and environment stress (4% CO2, 18% CO2) conditions. H. pylori cultured under environment stress displayed coccoid morphology and timedependent decrease in proliferation. We have further compared the protein expression profiles of H. pylori under normal growing and environment stress conditions by a global proteomic analysis, which includes high-resolution 2-DE followed by matrix-assisted laser desorption/ionization time of flight and nanoelectrospray/tandem mass spectrometry. In total, 42 protein spots were found to be up- or down-regulated by more than 2-fold under environment stress conditions. Of the 42 protein spots processed, 27 spots were identified; they represented 19 genes, including 2 kinds of hypothetical proteins.

Keyword

Helicobacter pylori; Proteome; Environment stress

MeSH Terms

Atmosphere
Duodenal Ulcer
Gastritis
Helicobacter pylori*
Helicobacter*
Mass Spectrometry
Proteome*
Stomach Neoplasms
Proteome
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