J Korean Assoc Oral Maxillofac Surg.  2009 Oct;35(5):294-298.

VEGF-related Autocrine Growth in Periosteal-Derived Cells

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21), Korea. surbyun@gsnu.ac.kr
  • 2Clinical Research Institute, Gyeongsang National University Hospital, Korea.
  • 3Department of Biochemistry, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21), Korea.
  • 4Department of Oral and Maxillofacial Surgery, College of Medicine, Ulsan University, Korea.
  • 5Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Korea.

Abstract

PURPOSE
The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periostealderived cells.
MATERIALS AND METHODS
Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate.
RESULTS
The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells.
CONCLUSION
These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.

Keyword

Periosteal-derived cells; Vascular endothelial growth factor; Autocrine growth

MeSH Terms

Alkaline Phosphatase
Ascorbic Acid
Calcium
Cell Culture Techniques
Dexamethasone
Durapatite
Glycosaminoglycans
Homeostasis
Humans
Osteoblasts
Periosteum
Phosphotransferases
Protein Isoforms
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-2
Alkaline Phosphatase
Ascorbic Acid
Calcium
Dexamethasone
Durapatite
Glycosaminoglycans
Phosphotransferases
Protein Isoforms
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-2
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