J Korean Assoc Maxillofac Plast Reconstr Surg.
2008 May;30(3):217-224.
Evaluation of angiogenic phenotypes in cultured human periosteal-derived cells under high-dose dexamethasone
- Affiliations
-
- 1Department of Oral and Maxillofacial Surgery, Gyeongsang National University, School of Medicine and Institute of Health Sciences, Jinju, Korea. surbyun@nongae.gsnu.ac.kr
- 2Clinical Research Institute, Gyeongsang National University Hospital, Korea.
- 3Department of Biochemistry, Gyeongsang National University School of Medicine and Institute of Health Sciences, Korea.
- 4Department of Oral and Maxillofacial Surgery, College of Medicine, Ulsan University, Korea.
- 5Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Korea.
Abstract
- Angiogenesis plays an important role in bone development and postnatal bone fracture repair. Vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptors (VEGFRs) have been thought to be primarily involved in promoting angiogenesis. It is well known that VEGF and its receptors have been reported to play an important role in the regulation of the interaction between angiogenesis and osteogenesis during bone repair processes. Dexamethasone, a potent synthetic glucocorticoid, promotes phenotype markers of osteoblast differentiation, such as ALP and osteocalcin. It stimulates in vitro osteogenesis of human bone marrow osteogenic stromal cells. Dexamethasone has been reported to suppress VEGF gene expression in some cells. However, our previous study demonstrated VEGF quantification increased in a time-dependent manner in periosteal-derived osteogenesis under dexamethasone. So, the purpose of this study was to examine the angiogenic phenotypes in cultured human periostealderived cells under high-dose dexamethasone. Periosteal-derived cells were cultured using a technique previously described. After passage 3, the periosteal-derived cells were further cultured for 28 days in an osteogenic inductive culture medium containing ascorbic acid, beta-glycerophosphate and high-dose dexamethasone, We evaluated the expression of VEGF isoforms, VEGFR-1, VEGFR-2, and neuropilin-1. ALL VEGF isoforms (VEGF(121), VEGF(165), VEGF(189), and VEGF(206)) expression was observed by RT-PCR analysis. VEGFR-1, VEGFR-2 and neuropilin-1 expression increased up to day 14, particularly during the early stage of mineralization. Our results suggest the involvement of direct VEGFs/VEGFRs system on periosteal-derived cells during early mineralization phase under high-dose of dexamethasone. These also suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.
MeSH Terms
-
Ascorbic Acid
Bone Development
Bone Marrow
Dexamethasone
Durapatite
Fractures, Bone
Gene Expression
Glycerophosphates
Humans
Neuropilin-1
Osteoblasts
Osteocalcin
Osteogenesis
Phenotype
Protein Isoforms
Receptors, Vascular Endothelial Growth Factor
Stromal Cells
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-2
Ascorbic Acid
Dexamethasone
Durapatite
Glycerophosphates
Neuropilin-1
Osteocalcin
Protein Isoforms
Receptors, Vascular Endothelial Growth Factor
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-2
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