Mycobiology.
2012 Dec;40(4):236-243. 10.5941/MYCO.2012.40.4.236.
Anticancer and Immunopotentiating Activities of Crude Polysaccharides from Pleurotus nebrodensis on Mouse Sarcoma 180
- Affiliations
-
- 1Division of Life Sciences, University of Incheon, Incheon 406-840, Korea. tslee@incheon.ac.kr
- 2Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
- 3Department of Life Science, University of Seoul, Seoul 130-743, Korea.
- 4Department of Life Science, Dongguk University, Seoul 100-715, Korea.
- 5Department of Clinical Laboratory Science, Shinsung University, Danggin 343-861, Korea.
- 6Mushroom Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 369-873, Korea.
- 7Bio-Resource and Environmental Center, University of Incheon, Incheon 406-840, Korea.
- KMID: 1428536
- DOI: http://doi.org/10.5941/MYCO.2012.40.4.236
Abstract
- Pleurotus nebrodensis is an edible and commercially available mushroom in Korea. This study was conducted in order to evaluate the anticancer and immunopotentiating activities of crude polysaccharides, extracted in methanol, neutral saline, and hot water (hereafter referred to as Fr. MeOH, Fr. NaCl, and Fr. HW, respectively) from the fruiting bodies of P. nebrodensis. beta-Glucan and protein contents in Fr. MeOH, Fr. NaCl, and Fr. HW extracts of P. nebrodensis ranged from 23.79~36.63 g/100 g and 4.45~6.12 g/100 g, respectively. Crude polysaccharides were not cytotoxic against sarcoma 180, HT-29, NIH3T3, and RAW 264.7 cell lines at a range of 10~2,000 microg/mL. Intraperitoneal injection with crude polysaccharides resulted in a life prolongation effect of 11.76~27.06% in mice previously inoculated with sarcoma 180. Treatment with Fr. NaCl resulted in an increase in the numbers of spleen cells by 1.49 fold at the concentration of 50 microg/mL, compared with control. Fr. HW improved the immuno-potentiating activity of B lymphocytes through an increase in alkaline phosphatase activity by 1.65 fold, compared with control at 200 microg/mL. Maximum production of nitric oxide (14.3 microM) was recorded in the Fr. NaCl fraction at 200 microg/mL. Production of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) was significantly higher, compared to control, and IL-6 production was highest, in contrast to TNF-alpha, IL-1beta, and positive control, concanavalin at the tested concentration of the various fractions. Results of the current study suggest that polysaccharides extracted from P. nebrodensis have a strong anticancer effect and may be useful as an ingredient of biopharmaceutical products for treatment of cancer.
Keyword
MeSH Terms
-
Agaricales
Alkaline Phosphatase
Animals
B-Lymphocytes
Cell Line
Fruit
Immunomodulation
Injections, Intraperitoneal
Interleukin-1beta
Interleukin-6
Korea
Life Support Care
Methanol
Mice
Nitric Oxide
Pleurotus
Polysaccharides
Sarcoma
Sarcoma 180
Spleen
Tumor Necrosis Factor-alpha
Water
Alkaline Phosphatase
Interleukin-1beta
Interleukin-6
Methanol
Nitric Oxide
Polysaccharides
Tumor Necrosis Factor-alpha
Water
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