Mycobiology.
2012 Sep;40(3):181-188.
Immunomodulating and Antitumor Activities of Panellus serotinus Polysaccharides
- Affiliations
-
- 1Division of Life Sciences, University of Incheon, Incheon 406-840, Korea. tslee@incheon.ac.kr
- 2Department of Life Science, University of Seoul, Seoul 130-743, Korea.
- 3Department of Life Science, Dongguk University, Seoul 100-715, Korea.
- 4Mushroom Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong 369-873, Korea.
- 5Bio-Resource and Environmental Center, University of Incheon, Incheon 406-840, Korea.
Abstract
- This study was initiated in order to investigate the anticancer and immunomodulating activities of crude polysaccharides extracted in methanol, neutral saline, and hot water (hereinafter referred to as Fr. MeOH, Fr. NaCl, and Fr. HW, respectively) from the fruiting bodies of Panellus serotinus. Content of beta-glucan and protein in Fr. MeOH, Fr. NaCl, and Fr. HW extracts of P. serotinus ranged from 22.92~28.52 g/100 g and 3.24~3.68 g/100 g, respectively. In vitro cytotoxicity tests, none of the various fractions of crude polysaccharides were cytotoxic against sarcoma 180, HT-29, NIH3T3, and RAW 264.7 cell lines at the tested concentration. Intraperitoneal injection with crude polysaccharides resulted in a life prolongation effect of 23.53~44.71% in mice previously inoculated with sarcoma 180. Treatment with Fr. HW resulted in an increase in the numbers of spleen cells by 1.3 fold at the concentration of 50 microg/mL compared with control. Treatment with Fr. NaCl resulted in improvement of the immuno-potentiating activity of B lymphocytes by increasing the alkaline phosphatase activity by 1.4 fold, compared with control, at the concentration of 200 microg/mL. Among the three fractions, maximum nitric oxide (13.48 microM) was recorded at 500 microg/mL in Fr. HW. Production of tumor necrosis factor alpha, interleukin-1beta, and interleukin-6 was significantly higher, compared to the positive control, concanavalin A, at the tested concentration. Therefore, treatment with crude polysaccharides extracted from the fruiting body of P. serotinus could result in improvement of antitumor activity.
Keyword
MeSH Terms
-
Alkaline Phosphatase
Animals
B-Lymphocytes
Cell Line
Concanavalin A
Fruit
Injections, Intraperitoneal
Interleukin-1beta
Interleukin-6
Life Support Care
Methanol
Mice
Nitric Oxide
Polysaccharides
Sarcoma 180
Spleen
Tumor Necrosis Factor-alpha
Water
Alkaline Phosphatase
Concanavalin A
Interleukin-1beta
Interleukin-6
Methanol
Nitric Oxide
Polysaccharides
Tumor Necrosis Factor-alpha
Water
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