Int J Stem Cells.  2013 May;6(1):67-74.

Osteogenic Differentiation of Bone Marrow Stem Cell in Poly(Lactic-co-Glycolic Acid) Scaffold Loaded Various Ratio of Hydroxyapatite

• Kim H
• Kim HM
• Jang JE
• Kim CM
• Kim EY
• Lee D
• Khang G

Abstract

BACKGROUND AND OBJECTIVES
Hydroxyapatite has biocompatibility and bioactivity and similar to bone of in human body. The purpose of this study is to evaluate osteogenic differentiation of bone marrow stem cell (BMSC) in PLGA Scaffold added various ratio of hydroxyapatite (HAp).
METHODS AND RESULTS
PLGA and PLGA/HAp scaffold were prepared using solvent casting/salt-leaching method. BMSC was seeded on the PLGA and PLGA/HAp scaffold and the samples were cultured in 37degrees C incubator with 5% CO2 for 28 days. Alkaline phosphatase (ALP) was carried out to evaluate alkaline phosphatase activity at 1, 3, 7, 10 and 14 days. Alizarin Red S stating was performed to identify calcium in scaffold at 1, 7, 14, 21 and 28 days. Compressive strength was measured to evaluate mechanical property of scaffold. To confirm cell viability, MTT was carried out at 1, 3, 7, 14 and 28 days. RT-PCR was performed to verify specific marker expression of osteoblast and stem cell at 7, 14, 21 and 28 days.
CONCLUSIONS
Osteogenic differentiation of BMSC was confirmed through ALP, RT-PCR, and alizarin red S staining in this study. These results suggest that HAp helps osteogenic differentiation of BMSC.

Keyword

Hydroxyapatite; Poly(lactic-co-glycolic acid); Scaffold; Bone marrow stem cell; Osteogenic differentiation

MeSH Terms

Alkaline Phosphatase
Anthraquinones
Bone Marrow
Calcium
Cell Survival
Compressive Strength
Durapatite
Human Body
Incubators
Lactic Acid
Osteoblasts
Polyglycolic Acid
Seeds
Stem Cells
Alkaline Phosphatase
Anthraquinones
Calcium
Durapatite
Lactic Acid
Polyglycolic Acid